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1 | User Manual I | Users Manual | 3.98 MiB | June 11 2019 |
Instructions for use SLIDEVIEW VS200 Optical Microscope and Accessory Any copyrights relating to this manual shall belong to OlympusSoftImagingSolutionsGmbH. We at Olympus Soft Imaging Solutions GmbH have tried to make the information contained in this manual as accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any warranty of any kind, whether expressed or implied, as to any matter whatsoever relating to this manual, including without limitation the merchantability or fitness for any particular purpose. Olympus Soft Imaging Solutions GmbH will from time to time revise the software described in this manual and reserves the right to make such changes without obligation to notify the purchaser. In no event shall Olympus Soft Imaging Solutions GmbH be liable for any indirect, special, incidental, or consequential damages arising out of purchase or use of this manual or the information contained herein. No part of this document may be reproduced or transmitted in any form or by any means, electronic or mechanical for any purpose, without the prior written permission of OlympusSoftImagingSolutionsGmbH. All brands are Trademark or registered Trademark of their respective owners. Olympus Soft Imaging Solutions GmbH All rights reserved Version 510_InstructionsForUse_VS200_de_00_xxJuly2019 Original instructions Manufactured by OLYMPUS SOFT IMAGING SOLUTIONS GmbH Johann-Krane-Weg 39 48149 Mnster, Germany Phone: +49 251 - 798 00 0 Fax: +49 251 - 798 00 6060 Email: info@olympus-sis.com Contents 1 About this manual 1.1 Notes on qualifications 1.2 Intended use 1.3 Other applicable documents 1.4 Symbols in this documentation 2 Safety 2.1 Categories and symbols in the safety instructions 2.2 Caution labels 2.3 Caution labels on the product 2.4 General safety instructions 2.5 Safety instructions for the laser 2.6 Safety instructions for X-Cite Turbo and X-Cite Xylis 3 Hinweise zum Aufstellen 3.1 Betriebs- und Lagerbedingungen 4 Tools and accessories 5 Scope of supply 6 System diagram 6.1 Available fluorescence components 7 Trays 7.1 Tray description 7.2 VS200 Tray types 8 Specifications 9 Unboxing 9.1 VS200 Scanner 9.1.1 Mounting of handles - VS200 scanner 9.2 Transportation locks for the VS200 scanner 9.2.1 Removing transportation lock from stage 9.2.2 Removing transportation lock from transmitted light condenser
(BX3-UCD8A) 9.3 VS200 Loader 9.3.1 VS200 Trays 9.3.2 PC and monitor 9.4 VS200 loader transportation locks 9.4.1 Removing transportation lock from VS200 loaders tray hotel 9.4.2 Removing transportation lock from SCARA robot arm OLYMPUS VS200 System
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Instructions for use 7 7 7 8 8 9 9 9 9 10 13 13 14 14 16 17 20 20 22 22 22 24 26 26 26 26 27 28 30 32 32 32 33 34 10.1 Scanner 11.3 Monochrome camera 10 Mounting of components 11.3.1 Orientation of the monochrome camera 9.4.3 Removing transportation lock from SCARA robot arms coun-
terweight 11 Mounting fluorescence components 10.1.1 Objectives 10.1.2 Immersion Objectives 10.1.3 Phase Contrast (PH) objectives 11.2.1 U-FFWR (Motorized fast reflected light filter wheel) 11.2.2 Add or replace filter (U-FFWR) 11.2.3 U-FFWO T3 (Motorized fast observation filter wheel) 11.2.4 Add or replace filter (U-FFWO) 11.2.5 TV 1.0x adapter 35 37 37 37 38 39 40 40 11.1 IX3-RFACA and IX3-RFALFE 11.2 Fluorescence filter wheels or camera adapter for monochrome camera 45 45 47 49 51 52 52 53 56 57 57 58 58 59 60 61 62 62 64 65 66 70 72 72 72 73 75 75 11.7.1 U-FF filter cube (IX3) 11.7.2 Add or replace filter cube (IX3) 11.7.3 U-FDICT filter cube 11.7.4 X-Cite Turbo 11.4 X-Cite adapter 11.5 4x Objective 11.6 LED light source 11.7 Filter set 12.1 Left panel 12.2 Right Panel 12.3 Back panel 12.4 Top panel 15 Verbindung VS200 Scanner und VS200 Loader 15.1 Mechanical Connection VS200 scanner and VS200 loader 13 Cabling 14 Assembly of the loader housing 12 Assembly of the housing for scanner 14.1 Top panel 14.2 Back panel 14.3 Front panels OLYMPUS VS200 System
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Instructions for use 16 PC operating system language 17 VS200 ASW software setup 18 VS200 device configuration 18.1 Activate the motorized polarizer 18.2 Activate the VS200 liquid dispenser 18.3 Device settings - objectives 18.4 Device settings - filter 18.5 Manual device configuration 18.5.1 ORCA monochrome camera 18.6 Device customization 18.6.1 ORCA camera adjustments 18.6.2 Setup phase contrast (PH) observation method 18.6.3 Setup polarization (Pol) observation method 18.6.4 Create or adjust an observation method 18.7 VS200 LED lamp voltages 18.7.1 Orca Flash 4.0 voltages (%) 18.7.2 Orca Fusion voltages (%) 18.7.3 VS-304M voltages (%) 18.7.4 VS-264C voltages (%) 19 How to insert a slide into a tray 19.1 How to insert a tray into the VS200 scanner 19.2 Insert a tray into the VS200 loader 20 Calibrate VS200 using the Olympus Calibration Slide 20.1 Stage limits - Z Axis 20.2 Camera adapter calibration for iDS (VS-264C) camera 20.3 Camera-To-Stage Rotation 20.4 XY Objective Shift / Parfocality 20.5 Shading Correction (Brightfield) 20.6 Shading correction of the label area 20.6.1 Preparation of calibration slide 20.6.2 Shading correction for polarization (Pol) 20.7 Magnification Test Scan 21 Additional calibrations for a fluorescence system 21.1 Camera adapter 21.1.1 Camera adapter U-FFWO T3 21.2 Camera-To-Stage Rotation 21.3 Camera shift 77 78 85 86 86 87 88 89 90 90 91 92 94 95 97 97 97 98 98 99 99 100 101 103 106 110 112 115 123 123 131 136 138 139 139 144 146 OLYMPUS VS200 System
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Instructions for use 21.4 Lens Correction 21.5 Shading correction BFMono 21.6 Magnification Test Scan 21.7 Shading correction for fluorescence observation methods 21.8 Shading correction for darkfield (DFMono) 21.9 Shading correction for PhaseContrast (PH) 21.10 Channel-XY-Shift 22 Cleaning the system 22.1 Cleaning the scanner 22.2 Cleaning the loader 22.3 Cleaning the trays 22.4 Cleaning the X-Cite Turbo 22.5 Cleaning the immersion objective 23 Additional software installation 23.1 OlyVIA 23.2 VS200 ASW Desktop 23.3 NetImage Server SQL (NIS-SQL) and Webinterface 24 Troubleshooting 24.1 Hardware not available 24.2 "No camera" error 24.3 Tray not active 24.4 Image too bright 24.5 Setting the Koehler illumination 25 Proper selection of the power supply cord 26 Declarations of conformity and disposal 26.1 CE Conformity (Europe) 26.2 WEEE declaration (Europe) 26.3 RoHS Conformity (Europe) 26.4 FCC conformity (USA) 26.5 For Korea only 26.6 China RoHS conformity (China) 26.7 RFID 26.7.1 RFID (Korea) 26.7.2 RFID (USA) 26.7.3 RFID (Canada) 26.7.4 RFID (Taiwan) 27 Support 150 153 158 160 164 167 171 174 174 174 175 175 175 178 178 178 178 179 179 179 179 180 180 186 189 189 189 189 189 190 190 190 190 190 191 191 192 OLYMPUS VS200 System
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Instructions for use 1 About this manual 1 About this manual This manual for the VS200 system is for Olympus technicians as well as users of the VS200 system who have been trained by Olympus. The manual contains important information on how to operate the VS200 system safely and correctly. In addition, this manual describes how to unbox and install the system as well as how to install further components. These tasks must expressly only be performed by Olympus. As a user you are not allowed to unbox or install the VS200 system by your-
self!
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this product, study this manual thoroughly before installing this product, and always keep this manual at hand when operating this product. Retain this instruction manual in an easily accessible place near the work area for future reference. Installing and configuring a liquid dispenser, and upgrading to a VS200 loader are not in the scope of this document. These must only be installed and adjusted by technical service of Olympus. 1.1 Notes on qualifications Olympus personnel Instructions for tasks that must only be performed by Olympus are identified with the following note:
The units described below must be assembled and adjus-
ted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 1.2 Intended use The system is intended to be used for the acquisition of virtual slide images. The sys-
tem is intended to be used for research only. If the VS200 system is used in a manner not specified by this manual, the safety of the user may be at risk. In addition, the VS200 system may also be damaged. Always use the VS200 system according to this instruction manual. Olympus Soft Imaging Solutions GmbH takes no responsibility for damaged caused by unintended use. Foreseeable misuse The system is not intended to be used for primary diagnosis. OLYMPUS VS200 System
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Instructions for use 1 About this manual 1.3 Other applicable documents Familiarize yourself with all of the other manuals for the components of the system. Take special note of the safety instructions they contain. Symbols in this documentation 1.4 Tools required for performing the steps Preconditions for the subsequent steps OLYMPUS VS200 System
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Instructions for use 2 Safety 2 2.1 Safety If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the product may also be damaged. Always use the product as outlined in this instruction manual. Categories and symbols in the safety instructions The safety instructions in this manual use symbols and keywords that are divided into the following categories:
CAUTION This warning sign and the word CAUTION indicate dangerous situations that can lead to minor injury if ignored. ATTENTION The exclamation mark and the word ATTENTION indicate situations where irre-
parable damage to the product can occur if ignored. Useful tips and important information This symbol indicates useful notes, tips and important information. 2.2 Caution labels Indicates a non-specific general hazard. This warning sign indicates that there is a crush hazard. 2.3 Caution labels on the product Caution labels have been placed at positions where special care is required when handling and operating the system. Always pay attention to the caution labels. CAUTION Moving components can cause injury Im Inneren des VS200 Systems verfahren Komponenten. Gaps open and close. Hands and fingers can get crushed. Sobald das System mit der Stromversorgung verbunden ist, Haare, Finger, Hnde und andere Krperteile sowie Kleidung und Gegenstnde aus dem Inneren des VS200 Systems fernhalten. OLYMPUS VS200 System
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Instructions for use Position of caution labels 2 Safety U-FFWO Loader Im Bereich des Mikroskoptisches Nosepiece Flap door Flap door motor 2.4 General safety instructions Do not plug anything into or unplug anything from the ports when the system is connected to the power supply. The system is not intended to be used for general microscopy tasks. No guarantee is provided on an image file. For example, even if the following state is generated attributable to an operation error of an observer, malfunction of the device, a system failure including a potential one, or the like, or at the time of normal operation or normal movement, it will not be possible to perform image restoration or damage compensation. 1. Abnormal state of an image 2. Abnormal state of file property such as a file name and file date and time 3. Disappearance of an image Confirm, edit or save the image for backup through care of the observer. To be noted is that the electronic recording media such as USB memory, HDD and DVD-R have retention lives in general and there may be the case that a file saved may disappear in several years. Further, there is no authenticity or tamper-proof characteristic. Prevent from strong vibrations as they can impair the image quality. Regarding to 3-axes control unit for scanning stages and motorised focus drives, Item caution for use Installation prevent from strong vibrations and risk of explosion 1. 2. 3. Temperature prevent from direct sunlight or strong heat sources 4. Humidity prevent from splash water and moisture OLYMPUS VS200 System
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Instructions for use 2 Safety 5. Radiation prevent from magnetic or electromagnetic sources in close proximity. Prevent from radioactive contamination 6. Chemicals/ toxic substances prevent from substances that are corrosive, potentially infectious, toxic or otherwise hazardous to health Regarding Monitor 1. Do not use this product near water. 2. Do not place this product on an unstable cart, stand or table. If the product falls, it could be seriously damaged. 3. Slots and openings are provided for ventilation to ensure reliable oper-
ation of the product and to protect it from overheating. These openings must not be blocked or covered. The openings should never be blocked by placing the product on a bed, sofa, rug or other similar surface. This product should never be placed near or over a radiator or heat register, or in a built-in installation unless proper ventilation is provided. 4. Never push objects of any kind into this product through cabinet slots as they may touch dangerous voltage points or short-out parts that could result in a fire or electric shock. Never spill liquid of any kind onto or into the product. The system must not be used for primary diagnosis. Possible data loss or invalid hardware states when forcing application to shut-
down. The application has no means of resisting a "forced shutdown" (e.g. due to a forced "Windows Update" or application kill via the "Task Manager", power loss, or a computer reboot). The user is generally required to not initiate such a forced shutdown. If the application is forced to shut down without being prop-
erly closed anyway, the following problems may occur:
The system may not be able to save and restore the current status of the application (including any non-saved states / images / settings). It may be necessary for a service technician to check the system to make it operational again. Photobleaching possible The system has a fluorescence light source with high light intensity. Pho-
tosensitive samples might be photo-bleached if they exposed to high-intensity light for long times (e.g. 60s). This can happen when the sample is being viewed in live mode or if it is scanned repeatedly (e.g. 10 times) on the specimen sites that are auto-focused a lot of times. The photobleaching is often only faint and only visible if the 16bit display limits are set very narrow. In order to prevent this, it can be useful to create alternative observation methods with reduced light intensity in order to only use as much light as necessary and avoid "over-
loading" the sample with excitation light. OLYMPUS VS200 System
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Instructions for use 2 Safety Shading / unneven light intensity For specimens with very sparse samples with a lot of empty background and rather small fluorescence emittance(e.g. FISH samples), it can occur that images acquired with Fluorescence illumination show a variance in background illu-
mination ("shading"). If the product is being used in a network environment, note the following points. Make sure that the network has appropriate virus protection. The installation of operating system service packs, security patches or third party software updates (including runtime libraries) can cause malfunctions. If anti-virus software is running while you are acquiring images, it can lead to the loss of individual acquisitions.It can also slow down the acquisition pro-
cess or even cause it to be aborted altogether. OLYMPUS VS200 System
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Instructions for use 2 Safety 2.5 Safety instructions for the laser 1. The loader uses a laser sensor. Never remove the warning and caution labels on the product. The semiconductor laser for loader incorporated in this product it designated as a product of the following class. CLASS 1 LASER PRODUCT(IEC60825-1:2007 / IEC608251:2014) This product complies with 21 CFR 1040. 10 and 1040. 11 except for deviations pursuant to Laser Notice No. 56, dated June 24, 2007. 2. Never attempt to remove the cover using a tool. There is a risk of exposure to the internal strong laser beam as well as other malfunction or failure. 3. Do not look into the light source either directly or indirectly. Otherwise, eye inquires may occur. 2.6 Safety instructions for X-Cite Turbo and X-Cite Xylis The X-Cite Turbo light source is not available in all countries. Follow the safety precautions at all times during operation and maintenance of this product. Non-observance may result in eye injury or damage to the system. CAUTION Risk of injury to the eyes Do not look at operating lamp/LED as it can emit UV light. Never look into the light emitting end of the light guide. The light could severely damage the eye if the light is observed directly. Always make sure the liquid light guide and X-Cite adapter are securely attached to the VS200 system. This will minimize the risk of exposure to the UV light. If the light source has a malfunction please contact Olympus or Excelitas cus-
tomer support. If the light source is serviced always make sure that the power cord is disconnected. Place the unit onto a hard, stable surface and make sure the ventilation openings are not covered by something. OLYMPUS VS200 System
- 13 -
Instructions for use 3 Hinweise zum Aufstellen 3 Hinweise zum Aufstellen A fully equipped VS200 system weighs approx. 190kg. Consider this weight when selecting the work surface. Beachten Sie die Angaben zum Gewicht, wenn Sie das VS200 System bzw. die Komponenten anheben. See Specifications auf Seite 24. Die Aufstellflche mussfr eine Tragfhigkeit von mindestens 190kg ausgelegt sein. Place the VS200 system on a sturdy level table or bench, taking care not to block the ventilation slots of the scanner and loader. When setting up the VS200 system, ensure that there is a minimum distance of 30cm from the wall and 20cm from other devices. The devices must only be moved using the designated handles to avoid dam-
ages on the device and personal injuries. The system should not be repositioned by the end user. If the system has to be repositioned an Olympus sales representative has to be contacted first. 3.1 Betriebs- und Lagerbedingungen Positioning the system Elevation Ambient temperature Humidity Power supply voltage fluc-
tuation Pollution degree Installation category (over-
voltage category) Only use the system indoors and in a laboratory or laboratory-
like environment 2000m maximum The maximum permissible range for the ambient temperature during operation of the VS200 system is 12 to 28C. The maximum permissible humidity during operation of the VS200 system is 80% for temperatures up to 31C (88F, con-
densation free) decreasing linearly through 70% at 34C (93F) and 60% at 37C (99F). The power supply should not fluctuate by more than 10% of the nominal voltage. 2 (in accordance with IEC60664) II (in accordance with IEC60664) Space required for the system VS200 System without loader Without fluorescence With fluorescence Height 530mm 885mm Area of work surface (table) 1500mm x 800mm 1500mm x 800mm OLYMPUS VS200 System
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Instructions for use 3 Hinweise zum Aufstellen VS200 System with loader Without fluorescence With fluorescence Height 885mm 885mm Area of work surface (table) 1800mm x 800mm 1800mm x 800mm Storage conditions The maximum permissible range for the ambient temperature for storage of the VS200 system is
-25 to 55C. The maximum permissible relative humidity range for storage is 10% to 95%. The maximum permissible temperature change rate for storage is 30C/h. The maximum permissible atmospheric pressure for storage is 70 to 106 kPa. OLYMPUS VS200 System
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Instructions for use 4 Tools and accessories 4 Tools and accessories 2,5mm, 3mm with ball end, 4mm, 5mm hex keys OLYMPUS VS200 System
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Instructions for use 5 Scope of supply VS200 Single Tray and VS200 Multi Tray Loader 5 Scope of supply 1st pallet 2nd pallet Additional box VS200 loader Pallet VS200 base unit Housing panel left Housing panel right (different variant for single tray and multi tray loader) Housing panel back Housing back panel cover Housing panel top front Housing panel top back Housing beam splitter cover 4 handles to carry the system PLN2x UPLANXAPO20x 1 1x3 inch tray VS200 standard computer with mouse and keyboard HP TFT 27 inch Instruction manual Calibration slide v2.0 Power supply (not available in all countries) Optional hardware VS200 loader Housing panel back Housing panel top Housing back panel cover 4 1x4 inch tray Instruction manual OLYMPUS VS200 System
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Instructions for use 5 Scope of supply Fluorescence kit A Box Fluorescence kit B Box IX3-RFACA IX3-RFALFE UPFLN4x U-FF filter cube TV1XC U-FFWR X-Cite Xylis IX3-RFACA IX3-RFALFE UPFLN4x U-FF filter cube 2x Black-out filter 1x Glas filter U-FFWO T3 U-FFWR X-Cite Xylis Fluorescence kit C The Fluorescence kit C is not available in all countries. Box IX3-RFACA IX3-RFALFE UPFLN4x U-FF filter cube 2x Black-out filter 1x Glas filter U-FFWO T3 X-Cite Turbo Fluorescence kit D The Fluorescence kit D is not available in all countries. OLYMPUS VS200 System
- 18 -
Instructions for use 5 Scope of supply Box IX3-RFACA IX3-RFALFE UPFLN4x U-FF filter cube TV1XC X-Cite Turbo OLYMPUS VS200 System
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Instructions for use 6 System diagram 6 System diagram 6.1 Available fluorescence components The VS200 system can be ordered with four different fluorescence sets. Each set con-
tains a basic configuration plus individual components. Filter sets and cameras are not part of the configuration. Remark: Configuration C and D are not available in all countries. Basic configuration Component IX3-RFACA IX3-RFALFE UPFLN4X object-
ive U-FF, filter cube
(empty) A X X X X Individual components B X X X X C X X X X D X X X X OLYMPUS VS200 System
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Instructions for use 12435679VS20-BUVS200 Slide Scanner Base Unit for Single Tray Loading85VS20-POWSUPVS200 Power Supply4VS20-LIQDISOil dispenser System3VS20-CAM-COVERVS200 Intermediate Part to Cover the B/W Camera7VS20-BU-LVS200 Slide ScannerBase Unit for Loader8VS20-LOADERVS200 Slide Loader Unit10VS20-LOAD-UGVS200 Slide Loader Upgrade for Single TrayLoading Units102VS20-304MFL camera VS304MHAMAMATSU C13440-20CUHamamatsu Orca Flash 4.0 V3Hamamatsu Orca Fusion1VS20-FL-AFluorescence excitation filter wheel and white light LEDVS20-FL-BFluorescence excitation and emission filter wheels and white light LEDVS20-FL-CFluorescence emission filter wheels and switchable LEDVS20-FL-DFluorescence with switchable LED only6VS20-ST13VS200 SlideTray (1x 3)VS20-ST13X5VS200 SlideTray (set of 5 trays for 1x3 slides)VS20-ST23VS200 SlideTray (2x3)VS20-ST34VS200 SlideTray (3x4)VS20-ST45VS200 SlideTray (4x5)7Fluorescence CamerasFluorescence SolutionAdditional Tray SolutionsAdditional Objective SolutionsVS200 system diagramLoader OptionsStandard Setup Single Tray Loading UnitFront viewRear viewXAPO/SAPO Objective10XUPLXAPO10X40XUPLXAPO40X40XUPLXAPO40XO60XUPLXAPO60XO60XUPLSAPO60XS100XUPLXAPO100XO100XUPLSAPO100XSPH Objective4XUPLFLN 4XPH10XUPLFLN 10X2PH20XUPLFLN 20XPH40XUPLFLN 40XPH60XUPLFLN 60XOIPH100XUPLFLN 100XO2PH 6 System diagram A X X X Component TV1XC U-FFWR U-FFWO T3 Xcite Xylis (IR), whitelight LED Xcite Turbo, switchable LED Optional - Filter sets Component A B X X X B C X X C D X X D Recommended filter set single-band exciter multi-band emit-
ter single-band exciter single-band emit-
ter single-band exciter single-band emit-
ter multi-band emit-
ter Monochrome cameras A 0 Component VS304M Orca Flash 4.0 ORCA Fusion VS200 B 0 0 0 C 0 0 0 D 0 0 0 OLYMPUS VS200 System
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Instructions for use 7 Trays 7.1 Tray description 7 Trays
(1)
(2)
(3)
(4)
(5) Button to open/close the spring for inserting a slide Slide pocket Slide position Indicator for label area Indicator for tray insertion 7.2 VS200 Tray types The VS200 systems can be equipped with four different tray types for different slide formats. All trays can be either used with the VS200 ST (Single Tray Load) system or VS200 MTL (Multi Tray Loader) system. By default every VS200 sys-
tem is equipped with at least one tray for 6 1x3 inch slides
(76 26 mm, DIN ISO 8037-
1). OLYMPUS VS200 System
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Instructions for use 7 Trays Additional tray types 3 slides in 2x3 inch (76 x 52 mm) 1 slide in 3x4 inch (76,3 x 103 mm) 1 slide in 4x5 inch (76,3 x 128 mm) Add image OLYMPUS VS200 System
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Instructions for use 8 Specifications 8 Specifications Intended spe-
cimen VS200 Single Tray Load (STL) VS200 Multi Tray Loader
(MTL) Observable specimen Glass slide with cover glass Size of glass slide Standard tray: width: 25mm 26.5mm, length:
75mm 76.5mm, thickness: 0.9mm - 1.2mm Optional trays:
1) Width: 51mm 53mm, length: 75mm 76.5mm, thickness: 0.9mm - 1.2mm 2) Width: 100mm 102mm, length: 75mm 76.5mm, thickness: 0.9mm - 1.2mm 3) Width: 126mm 128mm (scanable area restricted to 107mm), length: 75mm 76.5mm, thickness: 1.1mm - 1.4mm Size of cover glass Thickness: 0.12mm - 0.17mm Optical frame Illuminator Built-in Koehler illumination for transmitted light Objectives Compatible objectives 2x, 4x, 10x, 20x, 30x, 40x, 60x and 100x, selected oil and silicon immersion objectives, Phase objectives Motorized stage Motorized XY stage with automatic control Focusing Motorized automatic control Fluorescence Observation Camera sys-
tem Brightfield Fluorescence Motorized fluorescence illuminator Motorized filter wheel LED Fluorescence light source (Excelitas X-Cite Turbo, X-Cite Xylis) 2/3 CMOS camera, 3.45m x 3.45m pixel size, high sensitivity, high resolution Olympus VS304 high sensitive, high resolution or Hamamatsu ORCA Flash4.0 V3 and Orca Fusion OLYMPUS VS200 System
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Instructions for use 8 Specifications VS200 Single Tray Load (STL) VS200 Multi Tray Loader
(MTL) Scan Image correction Shading correction, auto white balance, true color Capacity 6 slides maximum (VS200 single tray) 210 slides max-
imum (VS200 multi tray loader) Pixel resolution (color cam-
era) 20X (NA 0.80): 0.274m/pixel Options:
10X (NA 0.40): 0.548m/pixel 40X (NA 0.95): 0.137m/pixel 40XO (NA 1.40): 0.137m/pixel 60XO (NA 1.42): 0.091m/pixel 100XO (NA 1.45): 0.055m/pixel Scan time Software Environment Weight Operating Environment Power Power Consumption Approx. 80sec (20x objective, scan area 15mm x 15mm brightfield) Image format: vsi, JPEG, JP2, TIFF/zooming while scanning/annotations/automatic sample detec-
tion/
Z stack extended focus imaging/screen cap-
ture/step less zooming/synchronized multi-images display automatic stitching/slide loader control VS200 single tray BF: 69kg VS200 single tray FL: 77kg VS200 multi tray loader BF: 142kg (without trays) VS200 multi tray loader FL: 150kg (without trays) PC & Monitor: 16kg 1 tray: 0.6kg 35 trays (maximum): 21kg Temperature: 15 28 degree centigrade, humid-
ity: 30% 80% (non condensing) Power Supply Ratings Input Output 100-240 VAC; 50/60 Hz; 4 A 24VDC, 9,2 A, 221 W MAX. OLYMPUS VS200 System
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Instructions for use 9 Unboxing 9 Unboxing The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 9.1 VS200 Scanner The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 9.1.1 Mounting of handles - VS200 scanner The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. CAUTION Risk of injury and risk of device damage if components are dropped The scanner or other components might drop when you are moving them. Injury to your feet or damage to the device can result. Make sure that all handles for transportation are screwed in completely and tight. The system must be carried by two people. Two people are required to lift the system onto a table 1. Mount the four handles (2 on each side) like in the image below. 2. Lift the system with two persons onto a stable table. The main center of gravity is at the back of the system. 3. Make sure that there is a gap of 30cm between the backside of the system and the wall. 9.2 Transportation locks for the VS200 scanner The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. OLYMPUS VS200 System
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Instructions for use 9 Unboxing This chapter describes how to remove the transportation locks from the VS200 scan-
ner after shipping. ATTENTION The transportation locks must be remounted each time before the unit is trans-
ported. Make absolutely sure not to lose the transportation locks and the mounting material. ATTENTION Mgliche Gerteschden durch Inbetriebnahme mit montierten Trans-
portsicherungen!
Wird der Scanner in Betrieb genommen, ohne dass zuvor die Trans-
portsicherungen vollstndig demontiert wurden, kann es zu Gerteschden kommen. Demontieren Sie vor Inbetriebnahme des Scanners unbedingt alle Trans-
portsicherungen!
9.2.1 Removing transportation lock from stage The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Hex screwdriver (size 2.5mm) 1. Go to the right side of the scanner. 2. Remove the red transportation lock from the microscope stage. To do so, remove the 2 hex screws (size 2.5mm). Sideview OLYMPUS VS200 System
- 27 -
Instructions for use 9 Unboxing Viewfromabove Viewfrombelow 9.2.2 Removing transportation lock from transmitted light condenser
(BX3-UCD8A) The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. ATTENTION The transportation locks must be remounted each time before the unit is trans-
ported. Make absolutely sure not to lose the transportation locks and the mounting material. Hex screwdriver (2,5mm, 3mm) 1. Remove the six 2.5mm hex socket screws which hold the black tamper pro-
tection plate like shown in the image below. OLYMPUS VS200 System
- 28 -
Instructions for use 9 Unboxing 2. Remove the transportation lock from the transmitted light condenser. To do so, remove the 2 hex screws (size 3) indicated in the figure. 3. Pull the transportation lock forwards and down to remove it. OLYMPUS VS200 System
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Instructions for use 9 Unboxing 9.3 VS200 Loader The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. CAUTION Risk of injury and risk of device damage if components are dropped The loader or other components might drop when you are moving them. Injury to your feet or damage to the device can result. Make sure that all handles for transportation are screwed in completely and tight. The system must be carried by two people. Two people are required to lift the loader system onto a table. 1. Remove the outer cardboard packaging and take all small boxes out of the package. 2. Take the top panel, back panel and the two front panels out of the boxes. 3. Unpack the handles. 4. Take the top of the loader cardboard packaging off and remove the outer cardboard (it consists of two parts). 5. Open the foil at the top and push it downwards to the bottom (the bottom of the loader cardboard packaging can be unfolded) until you can access the two covers to access the threads for the lifting handles on each side. 6. Remove the covers to access the threads for the handles. 7. Mount the four handles (2 on each silde) like in the images below. OLYMPUS VS200 System
- 30 -
Instructions for use 9 Unboxing 8. Make sure that all handles for transportation are screwed in completely and tight. 9. Remove all blue tape stripes for the grounding flags as well as the tape that secures the front door. 10. Remove the tape at the bottom left corner and take out the bubble foil pocket which contains the cable for the loader connection. 11. Open the door and remove the six desiccant bags. OLYMPUS VS200 System
- 31 -
Instructions for use 9 Unboxing 12. Use two persons to lift the loader onto a very stable table to the right side of the VS200 scanner. The center of gravity is at the right backside of the sys-
tem. 13. Remove the handles and keep them as they might be needed in the future. ATTENTION The system should not be repositioned by the end user. If the system has to be repositioned an Olympus sales representative has to be contacted first. 9.3.1 VS200 Trays 1. Unbox the VS200 trays carefully and handle them with care. Avoid bending of a tray at any time. 2. Put the trays aside for later use. 9.3.2 PC and monitor The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 1. Take the PC out of it's cardboard packaging. Inside the PC cardboard packaging you find the mouse and keyboard as well. 2. Take the monitor out of it's cardboard packaging and put it onto the table. All necessary cabels are inside the cardboard packaging. 9.4 VS200 loader transportation locks The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. This chapter describes how to remove the transportation locks from the VS200 loader after shipping. OLYMPUS VS200 System
- 32 -
Instructions for use 9 Unboxing Overview-transportationlocks ATTENTION Mgliche Gerteschden durch Inbetriebnahme mit montierten Trans-
portsicherungen!
Wird der Loader in Betrieb genommen, ohne dass zuvor die Trans-
portsicherungen vollstndig demontiert wurden, kann es zu Gerteschden kommen. Demontieren Sie vor Inbetriebnahme des Loaders unbedingt alle Trans-
portsicherungen!
ATTENTION The transportation locks must be remounted each time before the unit is trans-
ported. Make absolutely sure not to lose the transportation locks and the mounting material. 9.4.1 Removing transportation lock from VS200 loaders tray hotel The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. OLYMPUS VS200 System
- 33 -
Instructions for use 9 Unboxing Transportationlock(1) ATTENTION The transportation locks must be remounted each time before the unit is trans-
ported. Make absolutely sure not to lose the transportation locks and the mounting material. Hex screwdriver (size 3mm) 1. Remove the red transportation lock from the VS200 loaders tray hotel. To do so, loosen the 4 hex screws (size 3 mm) indicated in the figure. 9.4.2 Removing transportation lock from SCARA robot arm The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. OLYMPUS VS200 System
- 34 -
Instructions for use 9 Unboxing Transportationlock(2)and(3) ATTENTION The transportation locks must be remounted each time before the unit is trans-
ported. Make absolutely sure not to lose the transportation locks and the mounting material. Hex screwdriver (size 3mm) 1. Remove the red two-part transportation locks from the SCARA robot arm. To do so, loosen the 8 hex screws (size 3 mm) indicated in the figure. 9.4.3 Removing transportation lock from SCARA robot arms coun-
terweight The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. OLYMPUS VS200 System
- 35 -
Instructions for use 9 Unboxing Transportationlock(4) ATTENTION The transportation locks must be remounted each time before the unit is trans-
ported. Make absolutely sure not to lose the transportation locks and the mounting material. Hex screwdriver (size 3mm) 1. Remove the red transportation lock from the brass colored counterweight of the SCARA robot arm. To do this, loosen the 3 hex screws (size 3 mm) indicated in the figure. Make sure that the screws to do not fall inside the housing. The unit of SCARA robot arm and counterweight can now move freely. OLYMPUS VS200 System
- 36 -
Instructions for use 10 Mounting of components 10 Mounting of components 10.1 Scanner 10.1.1 Objectives The VS200 system is shipped with no objective mounted in the IX3-nosepiece. 1. Make sure that the power of the VS200 system is switched off!
2. Open the door of the VS200 scanner. 3. Make sure that the objective is clean and dust free before you mount it. 4. Depending on your VS200 kit you need to mount different objectives. The objectives should be mounted into fixed positions as per the list below. You can rotate the nosepiece revolver in any direction so that it is easier to mount an objective By default a VS200 system is shipped with a 2x and 20x objective. Additionally recommended objectives are the 10x and 40x UPLXAPO. Basically, all other Olympus objectives that fit into the IX3-nosepiece can be used and are supported. 5. Remove the small plastic protection cover from the objective position. 6. Screw in the objectives counter clockwise and make sure that the objective is screwed in tightly. OLYMPUS VS200 System
- 37 -
Instructions for use 10 Mounting of components Position list for objectives The position of the nosepiece is indicated at the center of the IX3-
nosepiece. Nosepiece position 1 2 3 4 5 6 Objective 2x PLN 20x UPLXAPO 10x UPLXAPO 40x UPLXAPO Any immersion objective or phase contrast objective 4x UPLFLN If you mount objectives in a different order you must subsequently adjust all device settings and observation methods. In this case refer to chapter Device settings - objectives auf Seite 87 and Device customization auf Seite 90. If you have a brightfield system only please continue with chapter Assembly of the housing for scanner auf Seite 62. 10.1.2 Immersion Objectives Pay attention to the following notes when using an immersion medium. CAUTION Certain immersion media can contain harmful substances. Make sure to read the manufacturer's safety data sheet before using your immersion medium. OLYMPUS VS200 System
- 38 -
Instructions for use 10 Mounting of components ATTENTION It is not possible to change the type of immersion medium once the glass laboratory bottle has been filled with either oil or silicone immersion medium. Otherwise damage can occur to the liquid dispenser. Before the VS200 system can be shipped (e.g. for repair) all liquids have to be removed from the system. Please contact the Olympus Customer Sup-
port for detailed information. It is not possible to change the type of immersion medium once the glass laboratory bottle has been filled with either oil or silicone immersion medium. Otherwise damage can occur to the liquid dispenser. As the liquid dispenser was tested with Olympus immersion media the best performance will be obtained with Olympus immersion media (Olympus Type-F Immersion Oil or Olympus Silicon Immersion Oil SIL300CS-30SC). The VS200 system can be equipped with an automatic liquid dispenser capable of dispensing different types of immersion medium. Once a particlular type of immer-
sion medium has been used, you can no longer switch to a different type. The liquid dispenser can only be installed by Technical Service of Olympus. The immersion objectives, oil or silicone, can be installed by anybody however. Mount the e.g. 60x UPlanXApo O objective into an empty position (e.g. pos. 5) of the IX3 nosepiece. Refer to chapter Device settings - objectives auf Seite 87 to configure this objective into the device settings. 10.1.3 Phase Contrast (PH) objectives The default configuration of a VS200 system includes 3 phase contrast inserts in the filter wheel of the BX3 condenser. BX3 condenser pos. 1 2 3 Insert U-PH1-S U-PH2-S U-PH3-S Mount e.g. the 20x UPlanFL N Ph1 objective into an empty position (e.g. pos. 5) of the IX3 nosepiece. Refer to chapter Device settings - objectives auf Seite 87 to include this objective into the device settings. OLYMPUS VS200 System
- 39 -
Instructions for use 11 Mounting fluorescence components 11 Mounting fluorescence components The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Skip this chapter if you have a brightfield system only. In the box with the fluorescence components (A, B, C or D) you find all parts needed to setup fluorescence for the VS200 system. Carefully unbox all components and fol-
low the instructions. 11.1 IX3-RFACA and IX3-RFALFE The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 3 mm and 4 mm hex key 1. Assemble the IX3-RFACA and IX3-RFALFE as shown in the image below and hand-tighten the two 3 mm hex socket screws at the junction. OLYMPUS VS200 System
- 40 -
Instructions for use 11 Mounting fluorescence components 2. Use a 3mm hex key to remove the two hex socket screws which hold the slider of the field stop. 3. Make sure that the field stop is pushed in completely. 4. Goto the scanner and open the front door. 5. Remove the six 2.5mm hex socket screws which hold the black tamper pro-
tection plate like shown in the image below. 6. Remove the two 3mm hex socket screws from the left and the right secur-
ing brackets from the VS200 system as shown in the image below. OLYMPUS VS200 System
- 41 -
Instructions for use 11 Mounting fluorescence components 7. Fold up the two securing brackets. 8. Remove the two 4mm hex socket screws from the RFAA supporting bracket, which is attached to the frame at the back of the system. 9. Insert the IX3-RFACA/ IX3-RFALFE unit into the system. Make sure that it fits into the rails on the left and the right side and flip down the brackets. OLYMPUS VS200 System
- 42 -
Instructions for use 11 Mounting fluorescence components 10. Tighten the two 3mm hex socket screws to fix the brackets. 11. Attach the RFALFE supporting bracket again at the back of the system's frame. Insert the two 4mm hex socket screws but don't tighten them. OLYMPUS VS200 System
- 43 -
Instructions for use 11 Mounting fluorescence components 12. Push the bracket upwards until it attaches to the IX3-RFALFE and tighten the two hex socket screws. 13. Make sure the power is switched off and attach the IX3-RFACA cable to the socket at the back of the IX3-RFACA. 14. Attach the black tamper protection plate again and tighten the six hex socket screws. OLYMPUS VS200 System
- 44 -
Instructions for use 11 Mounting fluorescence components Finally the system should look like on the image below. 11.2 Fluorescence filter wheels or camera adapter for mono-
chrome camera The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Depending on the customers configuration you either have to mount only one or two fast filter wheels. The following chapter describes how to mount the different fil-
ter wheels. 11.2.1 U-FFWR (Motorized fast reflected light filter wheel) The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. OLYMPUS VS200 System
- 45 -
Instructions for use 11 Mounting fluorescence components IMPORTANT Before you mount the U-FFWR make sure you insert all excitation filters into the filter wheel. Note the position of the individual filters as you might need them later for the observation method adjustment. If you mount them as set out in the table below, less adjustments will have to be made later. U-FFWR position 25 mm excitation filter 1 2 3 4 5 6 7 8 DAPI FITC CY3 CY5 CY7 Black-out filter Black-out filter Black-out filter Refer to the U-FFWR instruction manual to the chapter for filter insertion for more information. The orientation for Semrock emission filters is with the arrow towards the camera. The orientation for Chroma emission filters is with the arrow pointing away from the camera. 1. Attach the U-FFWR to the flange of the IX3-RFALFE at the back of the sys-
tem and tighten the two 3 mm headless hex screws. OLYMPUS VS200 System
- 46 -
Instructions for use 11 Mounting fluorescence components 2. Use the short CAN bus cable (35cm) to connect the U-FFWR to the VS200 system. Note the plug orientation indicated by the red dot. 3. In case you do not have a U-FFWO connect the terminator to the second plug at the U-FFWR. 11.2.2 Add or replace filter (U-FFWR) 1. Make sure that the power of the VS200 system is switched off. 2. Remove the backside top panel. See Top panel auf Seite 66. 3. To add or replace a 25mm emission filter in the U-FFWR open the cover of the filter wheel loosening the knurled head screw. OLYMPUS VS200 System
- 47 -
Instructions for use 11 Mounting fluorescence components 4. Insert the filter into an empty position. Make sure that you orient the filter correctly when you insert it. Refer to the U-FFWR instruction manual for more information. The correct orientation for Semrock emission filters is with the arrow towards the camera. The correct orientation for Chroma emission filters is with the arrow pointing away from the camera. 5. Write down the position number of the filter as you will need it later. 6. Configure the filter in the VS200 ASW software. To do this, open the
[Device Settings] dialog box.You can open this dialog box in the [Manual control] layout. 7. Click the [Previously Used Layout]
button to go to a different layout. You can find the [Previously Used Layout] button at the top right in the nav-
igation bar on the software's start page. 8. At the top right, on the menu bar click the [Manual control] button. 9. Select the [Acquire] >[Devices] > [Device Settings] command to open the
[Device Settings] dialog box. OLYMPUS VS200 System
- 48 -
Instructions for use 11 Mounting fluorescence components 10. Configure the filter. See Device settings - filter auf Seite 88. 11.2.3 U-FFWO T3 (Motorized fast observation filter wheel) The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Hex key (2.0) to lock the tubus Hex key (3.0) to open the filterwheel Small spanner to adjust the height of the tubus Spanner to fix the tubus 1. Before you can mount the U-FFWO T3 you need to assemble the bottom flange. The bottom flange is fixed with two 2.0mm hex socket screws. For details refer to the U-FFWO manual. OLYMPUS VS200 System
- 49 -
Instructions for use 11 Mounting fluorescence components Before you mount the U-FFWO T3, make sure you insert all emis-
sion filters into the filter wheel. Note the position of the individual filters as you might need them later for the observation method adjustment. Refer to chapter Add or replace filter (U-FFWO) auf Seite 51 for filter orientation. U-FFWO T3 position 25 mm emission filter 1 2 3 4 5 6 7 8 DAPI 432/36 FITC 515/30 CY3 595/31 CY5 680/42 CY7 Black-out filter Black-out filter 25 mm Glass 2. Refer to the U-FFWO instruction manual to chapter [Inserting the Optical Filters] for more information about filter insertion. 3. Mount the U-FFWO T3 with the cable connector facing to the left side onto the beam splitter and tighten the headless hex screw (3.0 mm) facing towards the front. OLYMPUS VS200 System
- 50 -
Instructions for use 11 Mounting fluorescence components 4. Connect the CAN-bus cable (which you find in the inside of the scanner) to the lower connector and the CAN-terminator to the upper connector. 11.2.4 Add or replace filter (U-FFWO) Hex key (3 mm) 1. Make sure that the power of the VS200 system is switched off. 2. To add or replace a 25 mm emission filter in the U-FFWO, open the cover of the filter wheel using a 3 mm hex key. 3. Insert the filter into an empty position. Make sure that you orient the filter correctly when you insert it. OLYMPUS VS200 System
- 51 -
Instructions for use 11 Mounting fluorescence components Refer to the U-FFWO instruction manual to chapter [Inserting the Optical Filters] for more information. The correct orientation for Semrock emission filters is with the arrow towards the camera. The correct orientation for Chroma emission filters is with the arrow pointing away from the camera. 4. Write down the position number of the filter as you will need it later. 5. Configure the filter in the VS200 ASW software. To do this, open the
[Device Settings] dialog box.You can open this dialog box in the [Manual control] layout. 6. Click the [Previously Used Layout]
button to go to a different layout. You can find the [Previously Used Layout] button at the top right in the nav-
igation bar on the VS200 ASW software's start page. 7. At the top right, on the menu bar click the [Manual control] button. 8. Select the [Acquire] >[Devices] > [Device Settings] command to open the
[Device Settings] dialog box. 9. Configure the filter. See Device settings - filter auf Seite 88. 11.2.5 TV 1.0x adapter The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 1. In case you have a TV1.0x adapter mount it onto the beam splitter and tighten the headless hex screw (3.0 mm) which is facing towards the front. 11.3 Monochrome camera The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. OLYMPUS VS200 System
- 52 -
Instructions for use 11 Mounting fluorescence components Depending on the fluorescence kit, VS200 offers three different monochrome cam-
eras:
VS304M (iDS) Orca Flash 4.0 (V3) ORCA Fusion VS200 When you switch on an Orca camera wait at least one minute before you start the VS200 ASW software otherwise the camera will not be recognized. If you have a TV1.0x adapter on top of the beam splitter, screw the camera on top of the adapter. See Orientation of the monochrome camera auf Seite 53 for the ori-
entation of the camera. 11.3.1 Orientation of the monochrome camera The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. The orientation of the monochrome camera is very important!
The orientation of the VS304M camera should be with the iDS logo to the front
(angled USB-C cable port to the back). The orientation of the ORCA Flash 4.0 camera should be with liquid cooling con-
nectors to the front (USB 3 and power connector on the left side). OLYMPUS VS200 System
- 53 -
Instructions for use 11 Mounting fluorescence components The orientation of the ORCA Fusion VS200 camera should be with the water cooling connectors facing the front side of the system. If your fluorescence kit contains a U-FFWO T3, follow the instructions below to mount a monochrome camera. For more details refer to the U-FFWO manuals. This example describes how to attach an ORCA Flash 4.0 camera to the U-FFWO T3. The attachment of VS304M (iDS) and ORCA Fusion VS200 is identical. Mounting a camera to the U-FFWO Refer to the U-FFWO instruction manual to chapter [Mounting a camera to the U-
FFWO] for more information. 1. Check that the counternut is pre-installed on the long side of the C-mount adapter. The counternut should be 1 turn away from the C-mount adapter.
(1)
(2)
(3)
(4) C-mount adapter counternut short side long side 2. Hand-tighten the short side of the C-mount adapter to the camera clock-
wise. OLYMPUS VS200 System
- 54 -
Instructions for use 11 Mounting fluorescence components
(1)
(2) camera C-mount adapter and counternut 3. Fasten the C-mount adapter (not the counternut) clockwise to the camera by using the large wrench tool as indicated below.
(1)
(2) fasten clockwise large wrench, small side 4. Hand-tighten the camera and C-mount adapter to the U-FFWO. Be aware to tighten the camera orientation lock first to prevent slippage of the locking mechansism. To do so, tighten the rota-
tion locking screw using a 2mm hex key. OLYMPUS VS200 System
- 55 -
Instructions for use 11 Mounting fluorescence components
(1)
(2)
(3) camera tighten rotation locking screw U-FFWO 5. As a final step, adjust orientation and parfocality of the camera, and then lock the camera via counternut to prevent accidential de-adjustment. How to adjust the camera adapter (tubus) is described in chapter Camera adapter auf Seite 139. 11.4 X-Cite adapter The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Hex key (3.0mm) X-Citeadapter OLYMPUS VS200 System
- 56 -
Instructions for use 11 Mounting fluorescence components 1. Depending on the system configuration attach the X-Cite adapter to either the IX3-RFALFE flange (A) directly, or - if a U-FFWR is present - attach it to the flange of the U-FFWR (B). OptionA:IX3-RFALFEflange OptionB:U-FFWR 11.5 4x Objective For proper system performance when acquiring fluorescence overview images the UPFLN4X has to be mounted on position 6 of the IX3-nosepiece. See Mounting of components auf Seite 37 for installation instructions. 11.6 LED light source The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Two LED light sources (X-Cite Xylis and X-Cite Turbo) are available for the VS200 sys-
tem. The X-Cite Turbo light source is not available in all countries. OLYMPUS VS200 System
- 57 -
Instructions for use 11 Mounting fluorescence components Follow the safety precautions at all times during operation and maintenance of this product. Non-observance may result in eye injury or damage to the system. CAUTION Risk of injury to the eyes Do not look at operating lamp/LED as it can emit UV light. Never look into the light emitting end of the light guide. The light could severely damage the eye if the light is observed directly. Always make sure the liquid light guide and X-Cite adapter are securely attached to the VS200 system. This will minimize the risk of exposure to the UV light. If the light source has a malfunction please contact Olympus or Excelitas cus-
tomer support. If the light source is serviced always make sure that the power cord is disconnected. Place the unit onto a hard, stable surface and make sure the ventilation openings are not covered by something. 1. Unbox the LED light source and avoid bending or stretching of the liquid light guide. 2. Mount the light guide into the X-Cite adapter and tighten the knurled head screw. Image Insert the other end of the light guide into the LED light source (see man-
ufacturers operating manual for detailed information). 3. 11.7 Filter set The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Depending on the purchased filter set insert the single-band excitation filters into the U-FFWR filter wheel. Refer to chapter Fluorescence filter wheels or camera adapter for monochrome camera auf Seite 45 for the position information. Insert the single-band emission filters into the U-FFWO (if available). Refer to chapter Fluorescence filter wheels or camera adapter for monochrome camera auf Seite 45 for position information. Put the 25mm glass filter into position 8 of the U-FFWO. Put the black-out filter into all empty filter wheel positions. The following chapters describe how to mount filter cubes into the IX3-RFACA. 11.7.1 U-FF filter cube (IX3) The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. OLYMPUS VS200 System
- 58 -
Instructions for use 11 Mounting fluorescence components 1. Open the door of the VS200 scanner. 2. Slide the door of the IX3-RFACA to the left to insert an IX3 filter cube. 3. Insert a new filter cube into the correct position (position 2) as shown in the image. The correct position is printed inside of the IX3-RFACA and is hard to read as the IX3-RFACA is mounted upside down. If you cannot read it make sure that the filter is placed between the position index 7 and 8 as shown in the image below. 4. After the cube is inserted slide the door of the IX3-RFACA back to the right. 11.7.2 Add or replace filter cube (IX3) 1. Make sure that the power of the VS200 system is switched off. 2. To add or replace an IX3 filter cube into the IX3-RFACA slide the door of the IX3-RFACA to the left. Insert the filter cube into an empty position. 3. 4. Write down the position number of the filter cube as you will need it later. 5. Configure the mirror cube in the VS200 ASW software. To do this, open the
[Device Settings] dialog box.You can open this dialog box in the [Manual control] layout. 6. Click the [Previously Used Layout]
button to go to a different layout. You can find the [Previously Used Layout] button at the top right in the nav-
igation bar on the software's start page. 7. At the top right, on the menu bar click the [Manual control] button. OLYMPUS VS200 System
- 59 -
Instructions for use 11 Mounting fluorescence components 8. Select the [Acquire] >[Devices] > [Device Settings] command to open the
[Device Settings] dialog box. 9. Configure the filter cube. See Device settings - filter auf Seite 88. 11.7.3 U-FDICT filter cube The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Mount an optional U-FDICT for polarized light acquisition. The U-FDICT should be placed into position 8 of the IX3-RFACA because this position is configured in the default device settings. 1. Insert the U-FDICT into position 8 as shown in the image. It is between the index 5 and 6. Refer to chapter Activate the motorized polarizer auf Seite 86 and Setup polarization (Pol) observation method auf Seite 94 to configure the system to scan with polarized light. If you would like to install other filter cubes please make sure that you register them in the dialog box [Device Settings] later. See Device settings - filter auf Seite 88. OLYMPUS VS200 System
- 60 -
Instructions for use 11 Mounting fluorescence components 11.7.4 X-Cite Turbo The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. The excitation filters in the X-Cite Turbo have to be placed as indicated in the boxes in the picture below (the arrows show the filter orientation). Refer to the X-Cite Turbo manual how to mount the filters. OLYMPUS VS200 System
- 61 -
Instructions for use 12 Assembly of the housing for scanner 12 Assembly of the housing for scanner Before starting with the assembly of the housing make sure the main power is disconnected!
The VS200 system is shipped with only the front door mounted. Side, back and top panels have to be attached on site. 1. Remove the four handles. Follow the correct order for mounting the elements of the housing so that you don't run into any trouble. 12.1 Left panel The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Before you install the left side panel make sure that the power switch of the U-CBM is switched on. OLYMPUS VS200 System
- 62 -
Instructions for use 12 Assembly of the housing for scanner The left side panel has two black quick lock pins (1) at the top and two rubber ring sockets (2) near the bottom. Additionally it has two connectors (3) for the grounding flags which need to be connected with the cables coming from the VS200 frame. On the left side of the VS200 scanner you find two quick locks sockets (1) at the top and two small pins (2) close to the bottom as well as two grounding flag connectors with cables attached (3). 1. Place the panel next to the system and connect the two grounding flags to the side panel first. 2. Mount the side panel so that the rubber rings of the panel fit onto the small pin on the VS200 system. 3. Once the pins are inside the rubber rings push the panel towards the frame and finally push it down so that the black quick lock pins of the side panel fit OLYMPUS VS200 System
- 63 -
Instructions for use 12 Assembly of the housing for scanner into the sockets. 12.2 Right Panel The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. Depending on your system configuration (with or without loader) the VS200 system comes with two different right side panels. The side panel of the loader variant has an opening for loading the trays. The mounting of the right side panel is similar to the left panel. There are two rubber sockets (quick locks at the top) and two small pins close to the bottom of the VS200 scanner and three grounding flags. 1. First connect the two grounding flags to the side panel. 2. Mount the side panel so that the rubber rings of the panel fit onto the small pin on the VS200 system. 3. Once the pins are inside the rubber rings push the panel towards the frame and finally push it down so that the quick lock pins of the side panel fit into OLYMPUS VS200 System
- 64 -
Instructions for use 12 Assembly of the housing for scanner the sockets. 12.3 Back panel The back panel needs to be attached before mounting the top panels. It is fixed with four knurled head screws. The three knurled head screws at the top are part of the top panel and are tightened later. 1. Guide the three cables from the backside of the VS200 system through the back panel like shown in the image below. OLYMPUS VS200 System
- 65 -
Instructions for use 12 Assembly of the housing for scanner 2. Guide the optional liquid light guide through the hole in the back like shown in the image below. 3. Place the back panel against the backside of the system and tighten the four knurled hex screws. 12.4 Top panel Top panel - first part The top panel consists of two parts. You have to start with the back part (large part) of the top panel. The panel has two quick lock pins (1) in the front and two guiding holes in the back (2). Additionally there are two grounding connectors (3) where you have to connect the grounding flags coming from the VS200 frame. On each side of the VS200 scanner there is a quick lock socket (1) and a small pin (2) as well as one connector with grounding flag attached (3). On each side there is the connector (system side) for the grounding flag. 1. Connect the two grounding flags (left and right side) to the top panel. 2. Put the top panel parallel to the scanner. OLYMPUS VS200 System
- 66 -
Instructions for use 12 Assembly of the housing for scanner 3. Gently push the top panel to the front of the system (where the front door is mounted). If the first part of the top panel is correctly mounted it should look like this:
Mount the beam splitter cover In case you have a brightfield system only you have to mount the beam splitter cover now. topview bottomview 1. Put the round cover on top of the beam splitter flange and fix it by tight-
ening the 3mm headless hex screw. Images Top panel - second part To attach the second part of the top panel follow the instructions below. OLYMPUS VS200 System
- 67 -
Instructions for use 12 Assembly of the housing for scanner On the VS200 scanner you find two quick lock sockets(1) and two quick lock pins (2). On the panel itself you find the corresponding parts; two quick locks sockets
(1) and two quick lock pins (2). 1. To make the assembly easier connect the two grounding flags already to the grounding plugs (3) of the top panel. 2. Open the front door and put the short top panel parallel on the scanner. 3. Gently push it towards the back of the system until the pins fit into the quick lock sockets. OLYMPUS VS200 System
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Instructions for use 12 Assembly of the housing for scanner 4. In case you use a U-FFWO make sure that the cable is guided through the housing like in the image below. If the top panels are mounted correctly, it should look like this:
5. Connect the two grounding flags to their counter plugs on the right and left side like shown in the image below. Leftside Rightside OLYMPUS VS200 System
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Instructions for use 13 Cabling 13 Cabling The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. The VS200 PC has a label on its backside indicating where to plug in the individual cables. It is important to connect all cables according to the connection scheme as otherwise components might not work correctly. Please refer to the following table:
Cable VS-364M (s/w) Color camera Orca Flash/Fusion Type USB 3.0 USB 3.0 USB 3.0 Location PC position Top of camera Back of VS200 Top of camera G H C DC Power In Power supply Back of VS200 Power socket USB 2.0 Tango USB 2.0 Back of VS200 A OLYMPUS VS200 System
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Instructions for use USB 2.0 Hub X-Cite Turbo X-Cite Turbo Xylis Xylis U-FFWR USB 2.0 USB 2.0 Power cord USB 2.0 Power cord CAN Bus 13 Cabling Back of VS200 Back of xCite Turbo I D Back of xCite Turbo Power socket Back of Xylis Back of Xylis VS200 internal D See chapter Fluor-
escence filter wheels or camera adapter for monochrome camera auf Seite 45. U-FFWO CAN Bus VS200 internal HP LCD screen Display port Bottom of LCD screen HP LCD screen Power cord Bottom of LCD screen Display port HP mouse HP keyboard USB 2.0 USB 2.0 E F OLYMPUS VS200 System
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Instructions for use 14 Assembly of the loader housing 14 Assembly of the loader housing The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. The VS200 loader is shipped with the two side panels already mounted. Follow the order of panel mounting as described in the chapter below. 14.1 Top panel The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 1. Place the top panel over the system. 1. Attach the two grounding flags at the backside of the loader to the con-
nectors of the panel like shown in the image below. 2. Position the panel so that it sticks out approximately 1.5 cm at the front. 3. Push it to the back until you hear a "click". 14.2 Back panel The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. The back panel is fixed with 8 knurled screws. 1. Center the back panel on the loader and start by tightening the middle knurled screws. OLYMPUS VS200 System
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Instructions for use 14 Assembly of the loader housing 2. Subsequently tighten all other knurled screws. 14.3 Front panels The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. 1. Mount the horizontal and vertical front panel as described in the following steps. Before After 2. Connect the two grounding flags to the horizontal panel and push the panel towards the system so that the quick lock sockets of the panel fit onto the quick lock pins of the loader. OLYMPUS VS200 System
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Instructions for use 14 Assembly of the loader housing 3. Connect the grounding flag to the vertical panel and push the panel towards the system so that the quick lock sockets of the panel fit onto the quick lock pins of the loader. OLYMPUS VS200 System
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Instructions for use 15 Verbindung VS200 Scanner und VS200 Loader 15 Verbindung VS200 Scanner und VS200 Loader 15.1 Mechanical Connection VS200 scanner and VS200 loader The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. This chapter describes the mechanical connection between the VS200 scanner and the VS200 loader. Hex screwdriver (size 5mm) 1. Switch the VS200 system off using the main power switch and disconnect the system from the power supply. 2. Place the VS200 loader to the right of the VS200 scanner, leaving a small gap between them. 3. Remove the handles from the right side of the VS200 scanner and from the left side of the VS200 loader. 4. Remove the jumper plug on the VS200 scanner. 5. Plug the VS200 loaders control cable into the port on the VS200 scanner where the terminator was. The plug should point towards the back. OLYMPUS VS200 System
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Instructions for use 15 Verbindung VS200 Scanner und VS200 Loader 6. Push the VS200 loader onto the connector on the VS200 scanner. 7. Tighten the 2 hex screws (size 5 mm) which attach the connector of the VS200 scanner to the VS200 loader. 8. Remove the remaining handles from the VS200 scanner and the VS200 loader. 9. Reconnect the VS200 system to the power supply. OLYMPUS VS200 System
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Instructions for use 16 PC operating system language 16 PC operating system language If you want to change the language of the operating system follow the instructions below. Japanese and Chinese are pre-installed on the VS200 PC. 1. Open the Windows Settings and select [Time & Language]. 2. Click on the language you would like to use. OLYMPUS VS200 System
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Instructions for use 17 VS200 ASW software setup 17 VS200 ASW software setup The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. This chapter describes the installation of VS200 ASW on a VS200 system PC. 1. Turn on the PC. 2. To start the setup process, go to D:\OLYMPUS_SERVICE_ONLY_DO_NOT_ DELETE\SetupMain\ and run the Setup.exe. 3. Click [Yes] to allow changes to the PC device. 4. Select the [Install or maintain imaging software] option and proceed with
[Next]. OLYMPUS VS200 System
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Instructions for use 17 VS200 ASW software setup 5. To provide the licenses open the envelope of the license cards and enter the license keys. You might have more than one license key depending on the configuration of your kit. Available license keys are:
VS200 ASW 3.1 VS-FLUO VS-CNV 6. Accept the license agreement. To activate the licences at customer site the PC needs internet access. If no internet access is available the licences can also be activated offline. In this case follow the instruction during the activation process. OLYMPUS VS200 System
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Instructions for use 17 VS200 ASW software setup 7. Change language (English is default). Proceed with [Next]. 8. Change installation location (not recommended). Proceed with [Next]. 9. A change of the temporary file location (D:\Temp) is not recommended. Proceed with [Next]. OLYMPUS VS200 System
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Instructions for use 17 VS200 ASW software setup 10. If your kit includes a different monochrome camera than the IDS-VS-304M select either ORCA-Flash4.0 or ORCA-Fusion VS200 and proceed with
[Next]. 11. Select [Add icon to the desktop] and [Add manuals shortcut to the desktop] and proceed with [Next]. 12. Start setup by clicking [Next]. OLYMPUS VS200 System
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Instructions for use 17 VS200 ASW software setup 13. Finish the setup process by clicking the [Finish] button. 14. In the following dialog box, click the [Close] button and do not install any example data or tools. 15. Turn on the VS200 system. OLYMPUS VS200 System
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Instructions for use 17 VS200 ASW software setup 16. To start the software double-click the VS200 ASW icon. 17. At the first start agree to the End-User License Agreement and proceed with [Continue]. The following splash screen is displayed while the VS200 system is ini-
tializing. The initialization process might need up to two minutes. Error messages If you see the error message below once the software is up and running please refer to Troubleshooting auf Seite 179. OLYMPUS VS200 System
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Instructions for use 17 VS200 ASW software setup In most cases this message occurs because you are using a wrong device con-
figuration. Refer to chapter VS200 device configuration auf Seite 85 to change or adapt the VS200 device configuration. If you see the following error message it means that you are using a VS200 loader which is not yet configured in the device list. Refer to chapter VS200 device configuration auf Seite 85 to add the loader to the system configuration. OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration 18 VS200 device configuration 1. On the start page of the software click the [Device Settings] button. The software will automatically switch to the [Manual control] layout and open the [Device Settings] dialog box. 2. In the [Device Settings] dialog box click on the [Device List] button to open the [Device List] dialog box. 3. VS200 ASW offers five predefined device configurations to choose from. Depending on the configuration of your VS200 kit (FL light source, fast filter wheels and filter set) select one of the five. If you have a VS200 brightfield system there is only one (VS200) device configuration available. OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration 4. If you are using the VS200 loader select the [Microscope] tab and select in the [Sample loading] list the [VS200 MTL Slide Loader] entry. 18.1 Activate the motorized polarizer All VS200 SLIDEVIEW systems are equipped with a polarization angle changer. By default the functionality of the motorized polarizer is switched off. 1. Use the [Acquire] >[Devices] > [Device List] command to open the [Device List] dialog box. 2. Select the [Transmitted light path] tab and select the [Polarization Angle Changer] check box. 18.2 Activate the VS200 liquid dispenser 1. Use the [Acquire] >[Devices] > [Device List] command to open the [Device List] dialog box. 2. Select the [Microscope] tab. 3. Make sure that the check box [VS200 Liquid Dispenser] is selected. OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration 18.3 Device settings - objectives A VS200 SLIDEVIEW kit is supplied with a 2x and a 20x objective ex-works. However, the predefined device configuration already contains a 2x, 4x, 10x, 20x and 40x objective. You need to delete the objectives that are not installed from the nosepiece settings in the [Device Settings] dialog box. Open the [Device Settings] dialog box 1. Use the [Previously Used Layout]
button to go to a different layout. You can find the [Previously Used Layout] button at the top right in the nav-
igation bar on the software's start page. 2. At the top right, on the menu bar click the [Manual control] button. 3. Select the [Acquire] >[Devices] > [Device Settings] command to open the
[Device Settings] dialog box. Configuring objectives 1. Select the [IX3 nosepiece] entry on the left side and set all objectives that are not installed to the status [Free]. Beispiel Before After 2. To add a phase contrast objective select the [Magnification] entry and the correct [Objective Type] entry for e.g. position 5 and add e.g. [PH] in the
[Description] field to distinguish between normal 20x and PH 20x. Beispiel OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration Refer to chapter Setup phase contrast (PH) observation method auf Seite 92 to adjust the [Device Customization] dialog box for phase con-
trast acquisition. 3. In case you want to install and configure an immersion objective please also check that the [Auto-Escape] function is checked. 4. In the [Device List] dialog box make sure that the [VS200 Liquid Dispenser]
check box is selected. See Activate the VS200 liquid dispenser auf Seite 86. 18.4 Device settings - filter If you add new hardware, like a new fluorescence filter, to the VS200 system you need to configure it in the [Device Settings] dialog box. Open the [Device Settings] dialog box 1. Use the [Previously Used Layout]
button to go to a different layout. You can find the [Previously Used Layout] button at the top right in the nav-
igation bar on the VS200 ASW software's start page. 2. At the top right, on the menu bar click the [Manual control] button. 3. Select the [Acquire] >[Devices] > [Device Settings] command to open the
[Device Settings] dialog box. Configuring filters 1. On the left of the tree structure, select the device into which you placed the filter. The following table shows the possible filter and device combinations. Filter type IX3 filter cube 25mm excitation filter 25mm emission filter VS200 device IX3 Mirror turret U-FFWR U-FFWO 2. In the [Pos.] options, select the position where you inserted the new filter. OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration 3. Type in the name of the filter in the [Filter] field. Example of filter configurations for U-FFWR and U-FFWO in the
[Device Settings] dialog box. 4. Add additional filters if required by selecting the appropriate position and selecting the name of the filter. 5. Click the [OK] button to save the changes. 6. Subsequently you have to configure an observation method in the [Device Customization] dialog box to be able to use the new filter. See Create or adjust an observation method auf Seite 95. 18.5 Manual device configuration This chapter describes the manual configuration of VS200 hardware devices. OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration 18.5.1 ORCA monochrome camera If your VS200 kit contains an ORCA Flash 4.0 or ORCA Fusion VS200 camera you need to manually add it to the device list as those two cameras are not part of the default setup. 1. Open the [Device List] dialog box and go to the [Camera] tab. 2. In the [Camera 2] list select either the ORCA Flash 4.0 or ORCA Fusion VS200. 3. Make sure that the correct camera adapter magnification (1.0x) is selected in the [Device Settings] dialog box. To open the [Device Settings] dialog box use the [Acquire] >[Devices] > [Device Settings] command. As none of the ORCA cameras are part of the default device configurations each observation method uses an ORCA camera needs to be adjusted manually. See Device customization auf Seite 90 for further information. 18.6 Device customization In the [Device Customization] dialog box you can make changes to existing obser-
vations methods. For example, you can add a different monochrome camera or you can add new observation methods. OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration 18.6.1 ORCA camera adjustments 1. Use the [Acquire] > [Devices] > [Device Settings] command to open the
[Device Settings] dialog box. 2. Select the [BFMono] observation method (OM). 3. Unlock the [BFMono] observation method by clicking on the lock icon. 4. 5. In the [Status] picklist, set the Orca camera to the status [Use]. In the [Image type] picklist, change the [Current] entry to the [16-bit gray-
scale] entry. 6. Do this for all other mono camera-related observation methods. OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration 7. Set the correct VS200 LED lamp voltages for the individual objectives. Refer to chapter VS200 LED lamp voltages auf Seite 97 for detailed information. 18.6.2 Setup phase contrast (PH) observation method If you want to add a phase contrast observation method do the following:
1. Select the [Acquire] >[Devices] > [Device Customization] command to open the [Device Customization] dialog box. 2. Select the [BFMono] observation method. 3. Use the [Copy Observation Method] button observation method. 4. Type in a name, e.g. "Phase Contrast". to make a copy of the 5. In the [Type] picklist select [Phase Contrast]. 6. Select the [BX3 Condenser] entry from the available components and choose the [Adjust per objective] entry in the [Status] pick list. OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration 7. Select the e.g. 20x phase contrast objective and select the [Use with this objective] check box. Refer to the following table for the correct assignment of the different phase contrast inserts to the correct magnification:
Objective magnification BX3 condenser PH insert 10x 20x 40x 60x 100x U-PH1-S U-PH1-S U-PH2-S U-PH3-S U-PH3-S 8. Select the [BX3 Top Lens] entry from the [Available components] and select the [Top Lens] > [In] option for the 20x PH objective. Refer to chapter VS200 LED lamp voltages auf Seite 97 for the correct lamp voltages for phase contrast acquisition. OLYMPUS VS200 System
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Instructions for use 18.6.3 Setup polarization (Pol) observation method If you want to add a polarization observation method do the following:
18 VS200 device configuration 1. Select the [Acquire] >[Devices] > [Device Customization] command to 2. open the [Device Customization] dialog box. In the [Device Customization] dialog box select the [BF] observation method. 3. Use the [Copy Observation Method] button observation method. 4. Type in a name, e.g. Polarization. to make a copy of the 5. In the [Type] picklist select [Polarized]. 6. Select the [IX3 Mirror Turret] entry from the available components and choose the [Adjust] entry in the [Status] pick list. In the [All objectives] tab select the [U-FDICT] analyzer for all objectives. 7. OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration 8. Select the [Polarization Angle Changer] entry from the available com-
ponents and choose the [Adjust] entry in the [Status] pick list. 9. Select the [BX3 Polarizer] and set the Status to [Use]. 10. Use the slide control to rotate the motorized polarizer or enter a defined angle value in the field. 18.6.4 Create or adjust an observation method The easiest way to create a new observation method (OM) e.g. for fluorescence image acquisition is to copy an existing one and adjust the settings according to your needs in the [Device Customization] dialog box. Open the [Device Customization] dialog box 1. Use the [Previously Used Layout]
button to go to a different layout. You can find the [Previously Used Layout] button at the top right in the nav-
igation bar on the VS200 ASW software's start page. 2. At the top right, on the menu bar click the [Manual control] button. 3. Use the [Acquire] > [Devices] > [Device Settings] command to open the
[Device Settings] dialog box. OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration Configuring an observation method 1. Select an observation method (e.g. CY3) in the [Name] list. This list con-
tains all of the observation methods that have already been defined. 2. Click the
[Copy Observation Method] button to create a copy of the observation method. 3. Enter a name for the observation method in the [Enter a New Observation Method Name] dialog box. In the [Available Components] list select the [Fluorochromes] entry. 4. 5. Select the [Use] entry from the [Status] list. 6. In the [Fluorochrome] list select the fluorochrome that fits to your new filter combination. In the [Available Components] list select the [ODB U-FFWO] entry. 7. 8. Select the new filter you have added. 9. Do the same if you e.g. added a new excitation filter for the ODB U-FFWR or a new IX3 filter cube in the IX3 Mirror Turret. 10. Click [OK] to save the changes. 11. As you created a new observation method you now have to carry out the OLYMPUS VS200 System
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Instructions for use 18 VS200 device configuration shading correction. See Shading correction for fluorescence observation methods auf Seite 160. 18.7 VS200 LED lamp voltages 18.7.1 Orca Flash 4.0 voltages (%) Objective 2x PLN 4x UPLFLN 10x UPlanXApo 20x UPlanXApo 40x UPlanXApo 40xUplanXApoS 60x UPlanXApoO 100x UPlanXApoO Observation method BFMono
(AS 75%) 0.6 0.6 0.8 0.5 0.7
-
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18.7.2 Orca Fusion voltages (%) Objective 2x PLN 4x UPLFLN 10x UPlanXApo 20x UPlanXApo 40x UPlanXApo 40xUplanXApoS 60x UPlanXApoO 100x UPlanXApoO Observation method BFMono
(AS 75%) 1.8 1.2 1.8 2.1 5.7 6.8 2.3 DFMono
(AS max) not defined
-
-
not defined not defined not defined not defined DFMono
(AS max) not defined 100 100 not defined not defined not defined not defined not defined PHMono
(AS 75%) not defined not defined
-
-
-
-
-
PHMono (AS 75%) not defined not defined 60 100 100 100 100 100 OLYMPUS VS200 System
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Instructions for use 18.7.3 VS-304M voltages (%) 18 VS200 device configuration Observation method BFMono
(AS 75%) DFMono
(AS max) PHMono
(AS 75%) Objective 2x PLN 4x UPLFLN 10x UPlanXApo 20x UPlanXApo 40x UPlanXApo 40xUplanXApoS 60x UPlanXApoO 100x UPlanXApoO 1.6 2.0 1.7 1.7 4.2
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-
18.7.4 VS-264C voltages (%) Objective 2x PLN 4x UPLFLN 10x UPlanXApo 20x UPLXAPO 40x UPLXAPO 40x UPlanXApoS 60x UPLXAPO 100x UPLXAPO Observation method BF
(AS 75%) 60 100 65 64 100 100 100 100 ND 100 100 not defined not defined not defined not defined DF
(AS max) not defined not defined not defined not defined not defined not defined ND ND
-
100 100 100 100 POL
(AS 75%) 100 100 100 100 100 100 100 100 OLYMPUS VS200 System
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Instructions for use 19 How to insert a slide into a tray 19 How to insert a slide into a tray 1. To insert a slide into a tray push the button on the tray away from the slide pocket to open. 2. Hold the button in place and put a slide into the slide pocket with the label facing the top as indicated on the tray [Label side]. 3. Push the slide to left side and release the button. 19.1 How to insert a tray into the VS200 scanner CAUTION Quetschgefahr beim Einsetzen der Trays in den VS200 Scanner Durch den motorisierten Antrieb der Einschubklappe am Scanner besteht Quetschgefahr. Hnde und Finger knnen beim Einsetzen der Trays gequetscht werden. Achten Sie darauf, dass keine Funktionen in der VS200 ASW Software aus-
gefhrt werden, whrend Sie den Einschub einsetzen. 1. To insert a tray into the VS200 scanner use the [Exchange Trays] function 2. of the VS200 ASW software to open the flap door. Insert the tray horizontally following the orientation which is printed on the tray: [Insert this way] into the rails and push it inside (about 6cm) until you cannot push it any further. OLYMPUS VS200 System
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Instructions for use 19 How to insert a slide into a tray 3. Click the [Close] button to close the flap door and the exchange dialog. 19.2 Insert a tray into the VS200 loader 1. To insert a tray into the VS200 loader use the [Exchange Trays] function of the VS200 ASW software to drive the loader into the loading/unloading position. Click the [Exchange Trays] button on the start page of the VS200 ASW. 2. Open the loader door and insert the tray horizontally following the ori-
entation which is printed on the tray ([Insert this way]) into an empty pos-
ition. 3. Push it all the way in until the tray indicator LED on the left side is green. 4. Close the loader door and click the [Lock Door] button in the VS200 ASW software. 5. Either use [Select Slide for Calibration] on the start page of the software to select a slide and tray for calibration or select any scan mode to select and load a tray into the scanner. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 20 Calibrate VS200 using the Olympus Calibration Slide This chapter describes how to calibrate a VS200 SLIDEVIEW System. 3mm hex key with ball end The VS200 ASW software offers a dedicated calibration wizard for all necessary cal-
ibrations. Theimageshowsthe[Calibrations]dialogbox. In order to get optimal results, we recommend using the VS Olympus Calibration Slide v2.0 to calibrate the system. Layout of the Calibration Slide (Version 2.0) The image shows the layout of the VS Olympus Calibration Slide v2.0. Always insert it with the label-area (left side) towards the defined label-area of the slide tray. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 1. Insert the VS-calibration slide into position 3 of the slide tray. 2. Click the [Exchange Trays] button on the start page of the VS200 ASW soft-
ware to insert the tray. 3. Click the [Select slide for calibration] button. 4. Load the tray (either manually or using the loader) by clicking the [Load Slide and Calibrate] button. The software switches to the [Manual control] layout. 5. In the [Manual control] layout select the [Acquire] > [Calibrations] com-
mand. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 20.1 Stage limits - Z Axis The X and Y axes do not have to be calibrated again. However, the Z axis calibration is dependent on the glass slide thickness. Therefore it is neces-
sary to calibrate the Z axis. 1. In the [Calibrations] dialog box select the [Stage Limits] entry and click the
[Calibrate] button. 2. Proceed with [Next] and follow the instructions of the wizard. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 3. Select the [Z Axis] check box and continue with [Next]. 4. At the top right in the menu bar click the [Manual control] entry to switch 5. 6. the layout. In the [Manual Control] layout, change to the 20x objective. In the [Camera Control] tool window click the [Live] button to switch to live mode. 7. Use the stage navigator to move to the center of the slide. 8. Use the [Ctrl] + mouse wheel combination to focus the sample. If you have trouble getting the image into focus, use a lower magnification first. 9. Once the image is in focus click the [Set Focus] button. Do not change the value for the Z-limit. The value should be 200. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 10. Click the [Finish] button to finalize the calibration process. OLYMPUS VS200 System
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Instructions for use
1 | User Manual II | Users Manual | 2.41 MiB | June 11 2019 |
20 Calibrate VS200 using the Olympus Calibration Slide 20.2 Camera adapter calibration for iDS (VS-264C) camera 3mm hex key with angle Standard Olympus 3mm hex key The iDS color camera is mounted and calibrated ex-works. The parfocality might change due to temperature changes. Therefore do not check this calibration with a cold system (e.g. directly after assembling the system). Wait at least 30 min with the system switched on to heat it up. If you use objectives with a cover slip correction collar it is recommended to adjust it prior to all following calibrations. The calibration process [Camera Adapter] also works with the 20x objective in case a 40x objective is not part of the system configuration. The standard for the focus distance is +/- 20m. However the closer you get to zero the better. Voraussetzungen In case your VS200 kit contains an objective with a cover slip correction collar ask the customer which coverslips they are using and note the thickness. Before you start with the camera adapter calibration make sure that the coverslip correction ring of the objective, for example 40x UPlanXApo, is set correctly. In most cases it should be in-between 0.15 0.17. Follow the instructions below to align the camera adapter. 1. In the [Calibrations] dialog box select the [Camera Adapter] entry and click the [Calibrate] button. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 2. The system will use the objective with the highest magnification and switch to live mode. If the image is not in focus click the [Autofocus] button to per-
form an autofocus. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 3. Select an area on the calibration slide were it is possible to focus with both the 40x and 2x objectives like in the screenshot below. 4. Click the [Next] button to continue. If the focus distance between the objectives differs too much, the wizard tells you to either shorten or stretch the camera adapter. 5. To adjust the distance, loosen the [LOCK] hex socket screw slightly and use the [FOCUS] hex socket screw to stretch or shorten the camera adapter. To turn the [FOCUS] screw use the angled hex key. If the value is positive, turn slightly clockwise. If the value is negative, turn slightly counter clockwise. 6. Tighten the [LOCK] screw. 7. Click the [Next] button in the [Camera Adapter Calibration] dialog box. 8. Repeat until the value meets the standard (+/- 20m or better). OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 9. Click the [Finish] button to finalize the calibration process. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 20.3 Camera-To-Stage Rotation The standard for the camera-to-stage rotation is +/- 0.1. However the closer you get to zero the better. 1. Select the [Camera-To-Stage Rotation] entry. 2. Click the [Calibrate] button to start the wizard. 3. Use the stage navigator to move to the center of the calibration slide as shown in the image below. 4. Select the 10x or 20x objective from the menu bar. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 5. If the value is beyond the acceptable rotation angle, loosen the headless hex screw used to fix the TV 0.63 adapter at the flange of the beam splitter. 6. After a slight rotation, tighten the screw again. If the value is negative, tighten the screw counter clockwise. If the value is positive, tighten the screw clockwise. Click the [Back] button in the window. Then click the [Autofocus] button in the next dialog box to perform the test again. 7. Adjust until the standard is met. 8. Finalize the process by clicking the [Finish] button. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 20.4 XY Objective Shift / Parfocality To carry out a proper XY shift/parfocality calibration use the position of the cal-
ibration slide shown in the screenshot below. 1. In the [Calibrations] dialog box select the [XY Objective Shift / Parfocality]
entry. 2. Click the [Calibrate] button to start the wizard. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 3. Select all objectives. Immersion objectives are calibrated in the same wiz-
ard. However the calibration wizard will calibrate all dry objectives first and subsequently select the immersion medium objective(s). 4. Make sure that the functions [Calibrate XY objective centering] as well as
[Calibrate Z parfocality] is selected. 5. Proceed with [Next]. 6. If the image seems not to be in focus perform an autofocus. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 7. The wizard will automatically select all of the objectives that are present and calculate the correction factors. If the Z parfocality for the 40x, 20x and 10x objectives is below 4m everything is fine. The Z parfocality distance for the 2x object-
ive should not exceed 20m. If the values do not match the standard please check whether all objective are screwed in completely and whether the calibration slide is clean. Repeat the calibration. 8. Finalize the calibration by clicking the [Finish] button. ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 20.5 Shading Correction (Brightfield) Use the stage navigator to move to the empty area of the calibration slide. The slide must be very clean (free of dust particles which will disrupt the shading correction procedure). 1. In the [Calibrations] dialog box select the [Shading Correction] entry and click the [Calibrate] button. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 2. Select the [BF] entry as the observation method and proceed with [Next]. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 3. When you carry out the calibration for the first time do not skip the acquis-
ition of the dark current correction image. For all future calibrations you can skip the acquisition of the dark current correction image. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 4. Proceed with [Next]. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 5. After the dark current correction image calibration, select all objectives. Immersion objectives are calibrated in the same wizard. However the cal-
ibration wizard will calibrate all dry objectives first and subsequently select the immersion medium objective(s). 6. Make sure that the options for [Shading correction] and [White balance]
are checked. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 7. Proceed with [Next]. The shading correction for the 2x and 4x objectives has to be done on an empty tray position. Use the stage navigator to move e.g. to position 4. Subsequently go back to the position where the calibration slide is inserted. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 8. Click the [Next] button to start the image acquisition process for the 2x objective. After the acquisition is complete, the calibration process automatically moves to the next objective. The shading correction for objectives with a magnification equal to or higher than 10x should be done on a sample glass slide with cover slip. You will receive good results if you use the VS-cal-
ibration slide. 9. Proceed with all other objectives in the same way. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 10. Click the [Finish] button to finalize the calibration process. ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 20.6 Shading correction of the label area 20.6.1 Preparation of calibration slide For the shading correction calibration of the label scan you need to prepare a proper slide. If a label printer is used, take an empty sticker and stick it on a normal glass slide as shown in the image below. If the customer does not use a label printer take a piece of white copy paper and glue it onto the slide. Example of a slide with an empty sticker 1. Insert the VS-calibration slide into position 3 of the slide tray and the pre-
pared label-shading-correction slide in position 2. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 2. Insert the tray by clicking the [Exchange Trays] button on the start page of the VS200 ASW software. If you use the loader put the tray in e.g. position 2. 3. Click the [Lock Door] button in the VS200 ASW software to lock the door. 4. Click the [Select Slide for Calibration] button on the start page of the VS200 ASW software. 5. Load the tray (either manually or using the loader) by clicking the [Load Slide and Calibrate] button. 6. In the [View] layout select the [Acquire] > [Calibrations] command. The [Calibrations] dialog box opens. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide Start the calibration 1. In the [Calibrations] dialog box select the [Shading Correction] entry and click the [Calibrate] button. 2. Select the [Label Scan] entry for the observation method and proceed with
[Next]. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 3. When you carry out the calibration for the first time do not skip the acquis-
ition of the dark current correction image. For all future calibrations you can skip the acquisition of the dark current correction image. Proceed with [Next]. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 4. Proceed with [Next] to start the acquisition process 5. Proceed with [Next]. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 6. After the dark current image calibration select all non-immersion object-
ives. Immersion objectives shall be calibrated separately. Proceed with
[Next]. IMPORTANT Actually, the label shading correction must only be performed for the 2x objective. However you should do it for all other objectives as well as otherwise there will always be an exclamation mark next to the entry. 7. Use the stage navigator to move e.g. to position 2 (where you placed the calibration slide for the label). Make sure you are in the center of the label sticker. 8. Click the [Next] button to start the image acquisition for the 2x objective. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 9. Focus on the empty label sticker and click the [Next] button to proceed. Use the focus up and down buttons like shown in the image below. After the acquisition is done the calibration process automatically moves to the next objective. 10. Proceed with all other objectives in the same way. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 11. Click the [Finish] button to finalize the calibration process. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 20.6.2 Shading correction for polarization (Pol) The result of the shading correction for polarization is dependent on the value for the [Polarization Angle Changer] which is set in the Polarization observation method. See Setup polarization (Pol) observation method auf Seite 94. If you change the angle you subsequently have to redo the shad-
ing correction. 1. In the [Calibrations] dialog box select the [Shading Correction] entry and click the [Calibrate] button. 2. Select the [Polarization] observation method. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 3. Skip the acquisition of the dark current correction image. 4. Select all objectives. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 5. Make sure that the options for [Shading correction] and [White balance]
are checked. The shading correction for the 2x and 4x objectives has to be done on an empty tray position. Use the stage navigator to move e.g. to position 4. Subsequently go back to the position where the calibration slide is inserted. 6. Click the [Next] button to start the image acquisition process for the 2x objective. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide After the acquisition is complete, the calibration process automatically moves to the next objective. The shading correction for objectives with a magnification equal to or higher than 10x should be done on a sample glass slide with cover slip. You will receive good results if you use the VS-cal-
ibration slide. 7. Focus on the part of the slide that contains the sample and subsequently move to a very clean area to acquire the shading image. 8. Proceed with all other objectives in the same way. 9. Click the [Finish] button to finalize the calibration process. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 20.7 Magnification Test Scan 1. In the [Calibrations] dialog box select the [Magnification Test Scan] entry and click the [Calibrate] button. 2. Now you can select whether you want to perform the magnification test scan for all objectives or only for certain ones. Immersion medium object-
ives are calibrated in the same wizard. However the calibration wizard will calibrate all dry objectives first and subsequently select the immersion medium objective(s). Select the [Use calibration slide] check box. OLYMPUS VS200 System
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Instructions for use 20 Calibrate VS200 using the Olympus Calibration Slide 3. Use the stage navigator to move to center (cross hair) of slide and auto-
focus. 4. Proceed with [Next]. 5. Click the [Finish] button to finalize the calibration process. In case the calibration value is not within the standard go back to the [Cam-
era-To-Stage Rotation] calibration and redo all calibrations. ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 21 Additional calibrations for a fluorescence system 1. Insert the VS-calibration slide into position 3 of the slide tray. 2. Click the [Exchange Trays] button on the start page of the VS200 ASW soft-
ware to insert the tray. 3. Click the [Select slide for calibration] button. 4. Load the tray (either manually or using the loader) by clicking the [Load Slide and Calibrate] button. The software switches to the [Manual control] layout. 5. In the [Manual control] layout select the [Acquire] > [Calibrations] com-
OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system mand. 21.1 Camera adapter The parfocality might change due to temperature changes. Therefore do not perform this calibration with a cold system (e.g. directly after assembling the system). Wait at least 30 min with the system switched on to heat it up. If you are using objectives with a cover slip correction collar, it is recom-
mended to adjust it prior to all subsequent calibrations. The limit for the focus distance is +/- 20m. However the closer you get to zero the better. 21.1.1 Camera adapter U-FFWO T3 This example describes the calibration for an ORCA Flash 4.0. The cal-
ibration for the VS-304M as well as the ORCA Fusion VS200 can be done in the same way. Small hex key to lock the tubus Hex key to open the filter wheel Small spanner to adjust the height of the tubus Spanner to fix the tubus The standard for the focus distance is +/- 20m. However the closer you get to zero the better. 1. In the [Calibrations] dialog box select the [Camera Adapter] entry and click the [Calibrate] button. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 2. Use [Ctrl] + mouse wheel to fine focus. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 3. Use the stage navigator to move to a similar position on the calibration slides to that shown in the image below. 4. Perform an autofocus. 5. If the focus differs too much you need to manually adjust the U-FFWO T3 adapter. Adjust the camera adapter 1. Release the camera rotation locking screw. Hold the camera with one hand to prevent camera rotation. Use the small wrench to adjust parfocality of the camera until the image on the screen is in focus. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system
(1)
(2)
(3) Release locking screw. Keep hold of camera to prevent rotation. Adjust parfocality. 2. Lock the parfocal setting by tightening the counternut. To do so, pull the large wrench clockwise to fasten the counternut while holding the small wrench steady.
(1)
(2) Hold steady. Fasten clockwise. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 3. Adjust the camera rotation by hand and tighten the camera rotation lock-
ing screw.
(1)
(2) Rotate camera. Tighten locking screw. 4. Execute an autofocus again and check the final result. 5. Repeat until the rotation value meets the standard. 6. Finalize the process by clicking the [Finish] button. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 21.2 Camera-To-Stage Rotation The standard for the camera-to-stage rotation is +/- 0.1. However the closer you get to zero the better. 1. In the [Calibrations] dialog box select the [Camera-To-Stage Rotation]
entry and click the [Calibrate] button. 2. Use the stage navigator to move to the center of the calibration slide. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 3. Select the 10x objective from the menu bar and perform an autofocus. 4. If the value is beyond the acceptable limits, loosen the screws in the beam splitter flange to rotate the U-FFWO together with the camera. 5. After a slight rotation, tighten the screws again and click the [Back] button in the dialog box. 6. Then click the [Autofocus] button in the next dialog box to perform the test again. 7. Repeat until the value meets the standard. 8. Finalize the process by clicking the [Finish] button. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 21.3 Camera shift The camera shift calibration corrects the shift between the brightfield and monochrome cameras. It is a software-based correction. Measure the camera shift between the two cameras 1. Open the [Manual control] layout. 2. Display the cross hair by selecting the [Cross Hair] entry in the [View]
menu. 3. Select the 20x objective. 4. Switch to live mode with the BF observation method selected. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 5. Use the stage navigator to move the stage so that the cross of the cal-
ibration slide is in the middle of the cross hair. 6. Fine focus with [Ctrl] + mouse wheel. 7. Switch off live mode and change to the BFMono observation method. 8. Switch on live mode again. You might recognize a shift. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 9. Switch to the [View] layout, go to the [Measurement and ROI] tab and select either the [Vertical Line] button or the [Horizontal Line] button. 10. Measure the shift between the cross hair and the center structure and note the X and Y values. In this case the monochrome camera is shifted up an to the left. To adjust the shift the cross hair needs to be shifted to the right (+X) and down (+Y). 11. Remember these values (+3.86m in X and +18.00m in Y) to enter them in the [Camera Shift] dialog box in the following steps. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 12. In the [Calibrations] dialog box select the [Camera Shift] entry and click the
[Edit] button. 13. In the [Camera Shift] dialog box be sure to select the correct objective (20x) and enter the values you measured manually to align the two cameras being used. 14. Click the [OK] button to finish the calibration process. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 21.4 Lens Correction On the calibration slide opposite the label area you find an area with check-
erboard grids in different sizes. Next to each grid there is a number (red circle in the example image) for the objective magnification from 4x to 60x/100x which has to be used for the calibration. 1. In the [Calibrations] dialog box select the [Lens Correction] entry and click the [Calibrate] button. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 2. Select all available objectives. Immersion are calibrated in the same wizard. However the calibration wizard will calibrate all dry objectives first and sub-
sequently select the immersion medium objective(s). 3. Use the stage navigator to move the VS calibration slide to the correct checkerboard area. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 4. Perform an autofocus and proceed with [Next]. 5. Do the same for all other objectives. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 6. Finalize the process by clicking the [Finish] button. If you see the message below please check if you are doing the calibration on the correct checkerboard area. ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38. 21.5 Shading correction BFMono 1. In the [Calibrations] dialog box select the [Shading Correction] entry and click the [Calibrate] button. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 2. Select the [BF Mono] observation method and proceed with [Next]. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 3. If you are calibrating an out of the box system (no shading correction was done before) start with the acquisition of the dark current correction image. 4. After the dark current image calibration select all objectives. Immersion objectives are calibrated in the same wizard. However the calibration wiz-
ard will calibrate all dry objectives first and subsequently select the immer-
sion medium objective(s). 5. Make sure the [Shading correction] check box is selected 6. Proceed with [Next]. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system IMPORTANT The shading correction for the 2x and 4x objectives has to be done on an empty tray position. Use the stage navigator to move e.g. to position 4. 7. Click the [Next] button to start the image acquisition process for the 2x objective. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system After the acquisition process is finished, the calibration process auto-
matically moves to the next objective. IMPORTANT The shading correction for objectives with a magnification equal to or higher than 10x should be done on a sample glass slide with cover slip. You will receive good results if you use the VS-cal-
ibration slide. 8. Proceed with all other objectives in the same way. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 9. Finalize the process by clicking the [Finish] button. ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38. 21.6 Magnification Test Scan 1. In the [Calibrations] dialog box select the [Magnification Test Scan] entry. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 2. Click the [Calibrate] button. The magnification test scan for the monochrome camera is identical with the magnification test scan for the color camera. See Magnification Test Scan auf Seite 136. 3. The resulting value should be within a deviation of +/- 0.1 degree. In case the calibration value is not within the standard go back to the [Cam-
era-To-Stage Rotation] calibration and readjust the rotation. This will also effect the shading correction, however. Refer to chapter Shading Cor-
rection (Brightfield) auf Seite 115 to redo the shading correction. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 21.7 Shading correction for fluorescence observation methods The shading correction for DAPI, FITC, CY3 and Cy5 can be done on a 'nor-
mal' thin, homogenous and large H&E sample using the autofluorescence like in the example below. However also fluorescence samples provided by the customer can be used for shading correction. To perform the shading correction for CY7 a real CY7 stained sample is necessary as CY7 does not show any autofluorescence. However shading correction is also sample dependent. Especially the thick-
ness of the sample might have an influence on the correction. It could be necessary to repeat the shading correction if different samples are used. 1. Put a similar slide into a tray and load the tray. 2. In the [Calibrations] dialog box select [Shading Correction] entry. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 3. Select e.g. the [Cy3] observation method and click [Next]. 4. Always skip the acquisition of the dark current correction image. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 6. 5. Select all objectives. Immersion objectives are calibrated in the same wiz-
ard. However the calibration wizard will calibrate all dry objectives first and subsequently select the immersion medium objective(s). It is very important to select in the [Calibration method] group the cal-
ibration method [Special]. Only the calibration method [Special] will work on a real sample. The calibration method [Standard] requires different samples and is less effective. Also make sure that the checkbox [Shading correction] is checked. 7. Proceed with [Next]. The system switches into live mode. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 8. Select an area on the slide large enough for a 2x field of view and focus the sample via Ctrl + mouse wheel and proceed with [Next]. For objectives equal or higher than 10x try to find a sample area which is quite homogenous. 9. Repeat these steps for all other selected objectives. 10. Continue the shading correction for all other fluorescence observation methods. ATTENTION In case you have calibrated an immersion medium objective as well clean the objective and remove immersion medium residues from the calibration slide. See Immersion Objectives auf Seite 38. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 21.8 Shading correction for darkfield (DFMono) The darkfield observation method can only be used with 4x and 10x objectives. To perform shading correction for DFMono use the checkerboard sec-
tions on the VS-calibration slide. 1. In the [Calibrations] dialog box, select the [Shading Correction] entry and click the [Calibrate] button. 2. Select the [DFMono] observation method and proceed with [Next]. 3. Skip the acquisition of the dark current correction image. Proceed with
[Next]. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 4. Select only the check boxes for the 4x and 10x objectives. Make sure that the [Shading correction] check box is selected. If necessary, select the [Calibration method] > [Standard] option. Proceed with [Next]. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system The system will activate the live mode with the 4x objective used. 5. Use the [Stage Navigator] tool window to navigate to the 20x check-
erboard and focus the sample. 6. Defocus by going down (-100 to 200m in Z) until you see a homogenous image (no checkerboards) anymore. In focus Defocused OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 7. Click the [Next] button to start the shading correction. 8. Continue in a similar way with the 10x objective. 9. For the 10x objective, go to the 100x/60x checkerboard area and defocus by going down 40m. 10. Click the [Finish] button to finalize the shading correction. 21.9 Shading correction for PhaseContrast (PH) Prepare a slide with some sample on it for focusing. The rest of the slide should be empty and very clean. In the [Calibrations] dialog box select the [Shading correction] entry. 1. 2. Click the [Calibrate] button. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 3. Select the [Phase Contrast] observation method. 4. Skip the acquisition of the dark current correction image. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 5. Only select the phase contrast objective(s). Make sure that the [Shading correction] check box is selected. 6. Focus on the part of the slide that contains the sample and subsequently search for a very clean area to acquire the shading image. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 7. Proceed with [Next]. 8. Finalize the process by clicking the [Finish] button. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 21.10 Channel-XY-Shift The channel-XY-shift calibration is only needed if multiple fluorescence fil-
ters or dichroic mirrors in the IX3-RFACA are used. As this calibration depends on the customer's specific system configuration and customer's samples, the Olympus employee installing the system should ask for a dedicated sample slide. If such a sample slide is not avail-
able, the customer itself can do the calibration at any time. It is important to correct a XY pixel shift induced by the different filter cubes. In the [Calibrations] dialog box select the [Channel-XY-Shift] entry. 1. 2. Click the [Calibrate] button. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 3. Select the [Calibrate transmission overlay observation methods] (bright field) check box. 4. Put in a customer fluorescent sample which contains stainings for all fluor-
escent filters that should be calibrated. Use at least the 20x objective, but preferably the 40x objective. 5. Navigate to an area with high contrast and focus. Then click the [Next] but-
ton to start the acquisition process. 6. Once the image has been acquired, the [Calibrate channel XY-shift] tool window opens. The shift between the individual channels is corrected manually here. The visibility of the channels can be changed by activating/deactivating the eye icon next to the channel name. 7. Click on a channel e.g. FITC to activate it and then use the cursor key to move the FITC image around pixel by pixel to match the structure of the underlying DAPI image. 8. Proceed with the other channels the same way. OLYMPUS VS200 System
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Instructions for use 21 Additional calibrations for a fluorescence system 9. When you have finished, click the [OK] button to save the changes. All calibrations should have a blue tick now. OLYMPUS VS200 System
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Instructions for use 22 Cleaning the system 22 Cleaning the system 22.1 Cleaning the scanner We recommend cleaning the scanner as required when you can see that it is dirty. ATTENTION Ingress of fluid can damage the devices. An ingress of fluid can cause a short circuit or damage the scanner, individual components of the scanner, the loader, and connected devices. Before cleaning the VS200 system, disconnect it from the power supply. Protect the scanner, the loader, and individual components from dirt, water and ingress of fluids. Clean only the surface of the scanner, the loader and the individual com-
ponents. 1. Disconnect the scanner from the power supply. 2. Clean the following components with a lint free cleaning cloth. The surface of the scanning stage Liquid dispenser head 3. For more pronounced marks, you can lightly wet the cleaning cloth with water or a mild solvent-free disinfectant or cleaning agent. 4. Clean the clamping mechanism only with a lint free cleaning cloth. 5. If the scanner or individual components were cleaned with a damp cloth, allow them to dry. 22.2 Cleaning the loader ATTENTION Ingress of fluid can damage the devices. An ingress of fluid can cause a short circuit or damage the scanner, individual components of the scanner, the loader, and connected devices. Before cleaning the VS200 system, disconnect it from the power supply. Protect the scanner, the loader, and individual components from dirt, water and ingress of fluids. Clean only the surface of the scanner, the loader and the individual com-
ponents. 1. Take all of the trays out of the loader. 2. Disconnect the VS200 system from the power supply. 3. Open the door of the loader. 4. Manually move the SCARA robot arm to the right. 5. Move the tray hotel to the loading position all the way at the front. OLYMPUS VS200 System
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Instructions for use 22 Cleaning the system 6. Clean the following components with a lint free cleaning cloth. Left side of the tray hotel Right side of the tray hotel The bottom panel of the tray hotel 7. For more pronounced marks, you can lightly wet the cleaning cloth with water or a mild solvent-free disinfectant or cleaning agent. 8. Push the tray hotel as far back as it will go to the back panel. 9. Manually move the SCARA robot arm to the left. 10. Clean the gripper only with a lint free cleaning cloth. 11. If the loader or individual components were cleaned with a damp cloth, allow them to dry. 22.3 Cleaning the trays 1. Clean the trays with a lint free cleaning cloth. For more pronounced marks, you can lightly wet the cleaning cloth with water or a mild solvent-free dis-
infectant or cleaning agent. 22.4 Cleaning the X-Cite Turbo The X-Cite Turbo light source is not available in all countries. Cleaning of optics should only be attempted by qualified personnel using appro-
priate fluids and lens paper. 1. To clean the exterior of the unit, use a slightly dampened cloth and a simple water/ detergent solution only. 22.5 Cleaning the immersion objective ATTENTION Damage to the objectives and hardware Objectives and hardware can get sticky after an immersion medium has been used.This can damage them. Clean the immersion objective after each use. CAUTION Quetschgefahr durch bewegte Komponenten im Inneren des VS200 Systems Im Inneren des VS200 Systems bewegen sich mechanische Komponenten. Wenn Sie bei eingeschaltetem System im Inneren des Systems hantieren, knnen Hnde und Finger gequetscht und Haare und Kleidung eingezogen werden. OLYMPUS VS200 System
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Instructions for use 22 Cleaning the system Achten sie darauf, dass Sie whrend der Reinigung der Immer-
sionsobjektive keine Softwarefunktionen ausfhren. 1. To clean lenses, remove dust by blowing them with a commercially avail-
able blower and wipe them lightly with cleaning paper (or a piece of repeatedly washed gauze). Only if they are stained by fingerprints or oils should you wipe them using cleaning paper slightly moistened with dehyd-
rated alcohol sold at store CAUTION Dehydrated alcohol is highly flammable. Do not expose it to heat or flame, and do not turn off or on the power switch of various elec-
trical apparatuses since ignition can be induced by just switching switches on and off. In addition, make sure that the room is well ventilated. 2. Do not use an organic solution to clean parts other than lenses. If a part is heavily stained, wipe it with a soft cloth slightly moistened with a diluted neutral detergent. Avoid cleaning lenses with the camera or the lenses removed. Doing so will cause the system to be uncalibrated. All calibrations need to be re-
done. 3. Click the [Clean Objective] button on the start page of the VS200 ASW soft-
ware and follow the instructions in the dialog box. The objective revolver will move up to grant more space. It is not necessary to unmount an objective to clean it. OLYMPUS VS200 System
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Instructions for use 22 Cleaning the system 4. In the [Select the objective to be cleaned] list select the objective to be cleaned. If you have more than one immersion objective, also select the other objectives in the list. 3. To clean the objective use lens cleaning paper. 4. After cleaning the objective click on the [Finish and set Objective status to'clean'] button. OLYMPUS VS200 System
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Instructions for use 23 Additional software installation 23 Additional software installation 23.1 OlyVIA To install OlyVIA go to D:\OLYMPUS_SERVICE_ONLY_DO_NOT_ DELETE\SetupOlyvia and execute the [setup.exe]. 23.2 VS200 ASW Desktop VS200 ASW and VS200 ASW DT cannot be installed on the same customer PC. The installation requires a VS200 ASW DT licence key during the installation. To install VS200 ASW DT on a different PC copy the setup files to the desired PC and execute the setup.exe. The setup files can be found on the VS200 ASW system PC under the follwing link: D:\OLYMPUS_SERVICE_ONLY_DO_NOT_ DELETE\SetupMain 23.3 NetImage Server SQL (NIS-SQL) and Webinterface The NetImageServer SQL is installed by Olympus Technical service only. Also the Webinterface to utilize OlyVIAweb (HTML5-based webviewer) is installed and configured by Olympus Technical Service only. OLYMPUS VS200 System
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Instructions for use 24 Troubleshooting 24 Troubleshooting 24.1 Hardware not available If the [IX3 Nosepiece:Hardware is not available] error message appears, do the fol-
lowing:
1. Close the VS200 ASW software. 2. Switch off the VS200 system!
3. Check cabling of IX3-RFACA. 24.2 "No camera" error If you see the following error messages after switching to the monochrome camera you need to check whether the correct camera is assigned in the observation meth-
ods which use a monochrome camera. See Manual device configuration auf Seite 89. In case you use an ORCA camera make sure that it is switched on. 24.3 Tray not active If you see the error message below you possibly inserted a new tray type without hav-
ing it set up correctly in the VS200 ASW software first. OLYMPUS VS200 System
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Instructions for use 24 Troubleshooting 1. Remove the tray and switch to the [Manual control] layout. 2. Use the [Acquire] > [Devices] > [Device Settings] command to open the
[Device Settings] dialog box. 3. Select the [VS200L Multi-Sample Holder] > [Tray Types] entry in the tree view. 4. Select the check box [Use Tray] next to the tray type you want to use. 24.4 Image too bright If you see the following error message please adjust VS200 LED lamp voltages. See ORCA camera adjustments auf Seite 91. 24.5 Setting the Koehler illumination The units described below must be assembled and adjusted by Olympus. If these units are assembled or adjusted by the customer, the operations are not ensured. This chapter describes how to set the Koehler illumination. Inbusschlssel (Gre 2,5mm und 3mm mit Kugelkopf) 1. Open the door of the VS200 scanner. 2. Remove the lower tamper protection plate. To do so, loosen the 5 hex OLYMPUS VS200 System
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Instructions for use screws (size 2.5 mm) indicated in the figure. 24 Troubleshooting 3. Use the main power switch to switch on the VS200 system. CAUTION Crush hazard when initializing the VS200 system Switching on the VS200 scanner and starting the VS200 ASW soft-
ware will initialize all components. As a result, various components begin to move. Gaps open and close when the stage moves. Hands and fingers can get crushed. Make sure that you are not within the stages range of move-
ment when it is moving. Try never to put your hands or fingers into any gaps. 4. Release the fixation in the field stop. Lsen Sie dazu die in der Abbildung markierten INBUS-Schrauben (Gre 3mm mit Kugelkopf). OLYMPUS VS200 System
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Instructions for use 24 Troubleshooting 5. Start the VS200 ASW software. 6. Take a tray with a slide in position 3. 7. On your softwares start page, click the [Exchange Trays] button to load the tray. 8. Use the [Load Slide for Calibration] function to load the slide. 9. Make sure that the 2x objective is selected. 10. Starten Sie das Live-Bild in der VS200 ASWSoftware. To do so, go to the 11.
[Manual Control] layout. In the [Camera Control] tool window, click the [Live] button to start the live image. Alternatively, you can also use the [F7] key. 12. Carry out an autofocus. 13. Change to the objective with the magnification 10x or 20x. 14. Carry out an autofocus again. 15. Turn the adjustment ring indicated in the figure to close the field stop as far as possible. You should now see a bright spot somewhere in the live image. The spot may appear polygonal (10 edges). Display a cross hair in the live image to help with centering. To do so, use the [View] > [Cross Hair] command in the VS200 ASW software. OLYMPUS VS200 System
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Instructions for use Alternatively, you can also use the [Alt + F6] keystroke. 24 Troubleshooting 16. Release the field stops focus setting. To do so, loosen the hex screw (size 3mm) indicated in the figure. 17. Focus the field stop. To do so, insert the hex key into one of the holes indic-
ated in the figure. Move the hex key to the right and to the left to adjust the focus ring. Set the focus so that the edges of the closed field stop are sharp. 18. Tighten the field stops focus setting. To do so, use the hex screw (size 3 mm) indicated in the figure. OLYMPUS VS200 System
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Instructions for use 24 Troubleshooting 19. Loosen the 2 hex screws (size 3mm) indicated in the figure. The screws should only have been screwed hand tight. 20. Use the two adjustment screws indicated in the figure to align the bright spot in the center of the cross hair. 21. Lock the field stops focus setting. To do so, tighten the hex screw (size 3 mm) loosened before. 22. Open the field stop completely. To do so, turn the adjustment ring shown in the figure to the left as far as possible. OLYMPUS VS200 System
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Instructions for use 24 Troubleshooting 23. Lock the field stop. To do so, tighten the hex screw (size 3 mm) indicated in the figure. 24. Close the VS200 ASW software and wait until the VS200 scanner has reached its end positions. 25. Use the main power switch to switch off the VS200 system. 26. Montieren Sie den schwarzen Eingreifschutz mittels der sechs Schrauben wieder. 27. Close the door of the VS200 scanner. OLYMPUS VS200 System
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Instructions for use 25 Proper selection of the power supply cord 25 Proper selection of the power supply cord If no suitable power supply cord has been provided, please select an appropriate power supply cord with a certification mark by referring to the specifications and the table below. Olympus is not responsible for damage caused by the use of uncer-
tified power cords with Olympus devices. Specifications Voltage Rating Rated current Temperature Rating Length Fittings configuration 125 V Wechselstrom (fr Gebiete mit 100-120V) oder 250V Wechselstrom (fr Gebiete mit 220-240V) min. 9,5A min. 60C max. 3,05m Kabel mit geerdetem Stecker. Gegenstck aufgeschweite Kup-
plung gem IEC-Konfiguration. OLYMPUS VS200 System
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Instructions for use 25 Proper selection of the power supply cord Table 1 - Certification marks for power cords The power cord has to have a certification mark from one of the bodies listed in table 1 or it must use a cable that has been tested by a body that is listed in table 1 or table 2. The plug has to have at least one certification mark shown in table 1. If you are unable to acquire a cord tested by one of the bodies listed in table 1 in your country, please use a cord that has been tested by a comparable body in your country. Country Agency Argentina IRAM Certification Mark Country Japan Agency JET, JQA Certification Mark Australia SAA Canada CSA Belgium CEBEC Netherlands KEMA Denmark DEMKO Norway NEMKO Germany VDE Austria VE Finland France United King-
dom Ireland Italy FEI UTE ASTA BSI NSAI IMQ Sweden SEMKO Switzerland Spain U.S.A. SEV AEE UL OLYMPUS VS200 System
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Instructions for use 25 Proper selection of the power supply cord Table 2 - HAR Flexible cables Certification body Printed or embossed harmonization mark (on the plug or cable insulation) Comit lectrotechnique Belge
(CEBEC) VDE Verband der Elektrotechnik Elektronik Informationstechnik e.V. CEBEC
<VDE>
Union Technique de llectricit
(UTE) USE Istituto Italiano del Marchio di Qualit (IMQ) British Approvals Service for Cables (BASEC) N.V. KEMA SEMKO AB Svenska Elektriska Materielkontroll-anstalten sterreichischer Verband fr Elektrotechnik (VE) Danmarks Elektriske Mater-
ielkontrol (DEMKO) National Standards Authority of Ireland (NSAI) Norges Elektriske Mater-
iellkontroll (NEMKO) Asociacin Electrotcnica Espaola (AEE) IEMMEQU BASEC KEMA-KEUR SEMKO
<VE>
<DEMKO>
<NSAI>
NEMKO
<UNED>
Hellenic Organization for Stand-
ardization (ELOT) ELOT Instituto Portugus da Qualidade
(IPQ) Schweizerischer Elektro-
technischer Verein (SEV) Elektriska Inspektoratet np SEV SETI
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
<HAR>
Underwriters Laboratories Inc. (UL) SV, SVT, SJ oder SJT, 3 X 18AWG Canadian Standards Association (CSA) SV, SVT, SJ oder SJT, 3 X 18AWG OLYMPUS VS200 System
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Instructions for use 26 Declarations of conformity and disposal 26 Declarations of conformity and disposal 26.1 CE Conformity (Europe) This system complies to the requirements of the following European directives:
Low Voltage Directive 2014/35/EU EMC Directive 2014/30/EU Machinery Directive 2006/42/EC Radio Equipment Directive (RED) 2014/53/EU This system complies with the requirements of Directive 2014/30/EU concerning elec-
tromagnetic compatibility according to Standard IEC/EN61326-1. Emission: Class A Immunity: Applied to industrial environment requirements. Operation of this equipment in a residential area may cause interference. 26.2 WEEE declaration (Europe) In accordance with the European directive on Waste of Electrical and Electronic Equipment, this symbol indicates that the product must not be disposed of as unsor-
ted municipal waste but should be collected separately. Refer to your local authority in the EU for return and/or collection systems available in your country. 26.3 RoHS Conformity (Europe) This product conforms with the European Union directive on the restriction of the use of certain hazardous substances in electrical and electronic equipment 2011/65/EU. 26.4 FCC conformity (USA) This device complies with Part 15 of the FCC regulations. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. This device has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful inter-
ference in a residential installation. This device generates, uses, and can radiate radio frequency energy. If not installed and used in accordance with the instruc-
tions, it may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular install-
ation. OLYMPUS VS200 System
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Instructions for use 26 Declarations of conformity and disposal If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encour-
aged to try to correct the interference by one or more of the following measures:
1. Reorient or relocate the receiving antenna 2. 3. Connect the equipment into an outlet on a circuit different from that to Increase the separation between the equipment and receiver which the receiver is connected 4. Consult the dealer or an experienced radio/TV technician for help FCC warning Changes or modifications not expressly approved by the party responsible for compliance could void users authority to operate the equipment. 26.5 For Korea only 26.6 China RoHS conformity (China) Delete this text and replace it with your own content. 26.7 RFID 26.7.1 RFID (Korea) 26.7.2 RFID (USA) FCC Supplier's Declaration of Conformity Hereby declares that the product Product name: Optical Microscope Model Number: VS200 Confirms to the following specifications:
FCC part 15, Subpart B, Section 15.107 and Section 15.109 Supplementary Information:
This device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. Responsible Party Name: Olympus Scientific Solutions Americas Corp. Address: 48Woerd Ave Waltham, MA 02453, U.S.A. Phone Number: 781-419-3900 Changes or modifications not expressly approved by the party responsible for com-
pliance could void the user's authority to operate the equipment. OLYMPUS VS200 System
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Instructions for use 26 Declarations of conformity and disposal FCC Part 15.19(a) [interference compliance statement], unless the following state-
ment is already provided on the device label:
This device complies with part 15 of the FCC Rules. Operation is subject to the fol-
lowing two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. 26.7.3 RFID (Canada) ISED notice:
This device complies with Industry Canadas licence-exempt RSSs. Operation is sub-
ject to the following two conditions:
(1) This device may not cause interference; and
(2) This device must accept any interference, including interference that may cause undesired operation of the device. Le prsent appareil est conforme aux CNR dIndustrie Canada applicables aux appar-
eils radio exempts de licence. Lexploitation est autorise aux deux conditions suivantes :
1) lappareil ne doit pas produire de brouillage;
2) lappareil doit accepter tout brouillage radiolectrique subi, mme si le brouillage est susceptible den compromettre le fonctionnement. 26.7.4 RFID (Taiwan) OLYMPUS VS200 System
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Instructions for use 27 Support Delete this text and replace it with your own content. 27 Support OLYMPUS VS200 System
- 192 -
Instructions for use Manufactured by OLYMPUS SOFT IMAGING SOLUTIONS GmbH Johann-Krane-Weg 39, 48149 Mnster, Germany Distributed by Shinjuku Monolith, 2-3-1, Nishi-Shinjuku, Shinjuku-ku, Tokyo 163-0914, Japan OLYMPUS CORPORATION OLYMPUS EUROPA SE & CO.KG Wendenstrasse 14-18, 20097 Hamburg, Germany OLYMPUS CORPORATION OF THE AMERICAS 3500 Corporate Parkway, P.O. Box 610, Center Valley, PA 18034-0610, U.S.A. OLYMPUS CORPORATION OF ASIA PACIFIC LIMITED L43, Office Tower, Langham Place, 8 Argyle Street, Mongkok, Kowloon, Hong Kong OLYMPUS AUSTRALIA PTY LTD 3 Acacia Place, Notting Hill, 3168, Australia TDO-MANUAL-VS200 - E97TDO-6122-00
1 | External Photos | External Photos | 370.61 KiB | June 11 2019 |
External Photos OLYMPUS VS200 Base Unit open/closed:
Loader Unit open/closed:
1 / 6 Front/left/right/back/top view front 2 / 6 left 3 / 6 right 4 / 6 top 5 / 6 back 6 / 6
1 | ID Label | ID Label/Location Info | 351.10 KiB | June 11 2019 |
Packaging Label 65x100mm:
Type Label with placeholders 70x44mm:
Label Position is on backside of the device:
1 | Agents Letter | Cover Letter(s) | 126.89 KiB | June 11 2019 |
OLYMPUS 10/30/2019 TUV SUD Product Service GmbH Aussere Fruehlingstrasse 45 94315 Straubing Deutschland / Germany Dear Sir or Madam, We, (Olympus Soft Imaging Solutions GmbH), hereby authorize TUV SUD Product Service GmbH, Aussere Fruehlingstrasse 45, 94315 Straubing, Deutschland / Germany to act as our agent in all matters relating to applications for equipment authorization, external lab testing, etc including the signing of all documents relating to these matters. We authorize TUV SUD Product Service GmbH during the processing of this project the right to subcontract further agents, national authorities, national test laboratories and to send documents and test samples to these agents, national authorities, national test laboratories and other national instances necessarily required for the approval process. This authorization expires on 07.08.2020. Sincerely, pw ye Head of Quality & Regulations Head of Fin:
Jennifer Hoffmann Jorg Sei Telephone Number: 0251-798006316 Jennifer.hoffmann@olympus-sis.com OLYMPUS SOFT IMAGING SOLUTIONS GMBH
-Krane-Weg 39, D-48149 Minster, PI Managing Directors: Michael Speiser (Exec. VATID: DE 813 446 409, Place ot s imber: 4221081013, SWIFT/BIC: BNPADEFFXXX, IBAN: DE13 5121 0600 4221 0810 13 BNP
1 | Confidentiality Letter | Cover Letter(s) | 131.87 KiB | June 11 2019 |
OLYMPUS 10/30/2019 TUV SUD BABT TCB Octagon House, Segensworth Road, Fareham, Hampshire, PO15 SRL Confidentiality Request FCC ID: 2AUAK-VS200 Pursuant to Sections 0.457(d)(1)(ii) and 0.459 of the Commissions Rules, OLYMPUS SOFT IMAGING SOLUTIONS GmbH, Johann-Krane-Weg 39, 48149 Munster, Germany hereby requests permanent confidential treatment of information accompanying this application as outlined below:
Schematics Bill of Materials/Parts List Block Diagrams Theory of Operation The above materials contain trade secrets and proprietary information not customarily released to the public. The public disclosure of these matters might be harmful to the Applicant and provide unjustified benefits to its competitors. The Applicant understands that pursuant to Rule 0.457(d)(1)(ii), disclosure of this Application and all accompanying materials will not be made before the date of the Grant for this Application. Yours sincerely, Yon f'L dpe Name: Jennifer Hoffmann Jorg Seitl Title: Head of Quality & Regulations Head of Finance & Controlling & IT OLYMPUS SOFT IMAGING SOLUTIONS GMBH
frequency | equipment class | purpose | ||
---|---|---|---|---|
1 | 2019-11-13 | 0.125 ~ 0.125 | DCD - Part 15 Low Power Transmitter Below 1705 kHz | Original Equipment |
app s | Applicant Information | |||||
---|---|---|---|---|---|---|
1 | Effective |
2019-11-13
|
||||
1 | Applicant's complete, legal business name |
Olympus Soft Imaging Solutions GmbH
|
||||
1 | FCC Registration Number (FRN) |
0028724524
|
||||
1 | Physical Address |
Johann-Krane-Weg 39
|
||||
1 |
Muenster
|
|||||
1 |
Germany
|
|||||
app s | TCB Information | |||||
1 | TCB Application Email Address |
a******@tuvsud.com
|
||||
1 | TCB Scope |
A1: Low Power Transmitters below 1 GHz (except Spread Spectrum), Unintentional Radiators, EAS (Part 11) & Consumer ISM devices
|
||||
app s | FCC ID | |||||
1 | Grantee Code |
2AUAK
|
||||
1 | Equipment Product Code |
VS200
|
||||
app s | Person at the applicant's address to receive grant or for contact | |||||
1 | Name |
J****** H******
|
||||
1 | Title |
Ms
|
||||
1 | Telephone Number |
+49(0********
|
||||
1 | Fax Number |
+49(0********
|
||||
1 |
j******@olympus-sis.com
|
|||||
app s | Technical Contact | |||||
n/a | ||||||
app s | Non Technical Contact | |||||
n/a | ||||||
app s | Confidentiality (long or short term) | |||||
1 | Does this application include a request for confidentiality for any portion(s) of the data contained in this application pursuant to 47 CFR § 0.459 of the Commission Rules?: | Yes | ||||
1 | Long-Term Confidentiality Does this application include a request for confidentiality for any portion(s) of the data contained in this application pursuant to 47 CFR § 0.459 of the Commission Rules?: | No | ||||
if no date is supplied, the release date will be set to 45 calendar days past the date of grant. | ||||||
app s | Cognitive Radio & Software Defined Radio, Class, etc | |||||
1 | Is this application for software defined/cognitive radio authorization? | No | ||||
1 | Equipment Class | DCD - Part 15 Low Power Transmitter Below 1705 kHz | ||||
1 | Description of product as it is marketed: (NOTE: This text will appear below the equipment class on the grant) | Slide scanner | ||||
1 | Related OET KnowledgeDataBase Inquiry: Is there a KDB inquiry associated with this application? | No | ||||
1 | Modular Equipment Type | Does not apply | ||||
1 | Purpose / Application is for | Original Equipment | ||||
1 | Composite Equipment: Is the equipment in this application a composite device subject to an additional equipment authorization? | No | ||||
1 | Related Equipment: Is the equipment in this application part of a system that operates with, or is marketed with, another device that requires an equipment authorization? | No | ||||
1 | Is there an equipment authorization waiver associated with this application? | No | ||||
1 | If there is an equipment authorization waiver associated with this application, has the associated waiver been approved and all information uploaded? | No | ||||
app s | Test Firm Name and Contact Information | |||||
1 | Firm Name |
TUV SUD Product Service GmbH
|
||||
1 | Name |
M****** B********
|
||||
1 | Telephone Number |
49942********
|
||||
1 | Fax Number |
+49 9********
|
||||
1 |
m******@tuev-sued.de
|
|||||
Equipment Specifications | |||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Line | Rule Parts | Grant Notes | Lower Frequency | Upper Frequency | Power Output | Tolerance | Emission Designator | Microprocessor Number | |||||||||||||||||||||||||||||||||
1 | 1 | 15C | 0.12500000 | 0.12500000 |
some individual PII (Personally Identifiable Information) available on the public forms may be redacted, original source may include additional details
This product uses the FCC Data API but is not endorsed or certified by the FCC