FCC ID: 2A6TQDNBSEQ-T7RS Genetic Sequencer

About the user manual This user manual is applicable to DNBSEQ-T7RS Genetic Sequencer. The manual version is 3.0 and the software version is V1. This manual and the information contained within are proprietary to Wuhan MGI Tech Co., Ltd. (hereinafter called MGI), and are intended solely for the contractual use of its customer in connection with the use of the product described herein and for no other purpose. Any person or organization can not entirely or partially reprint, copy, revise, distribute or disclose to others the manual without the prior written consent of MGI. Any unauthorized person should not use this manual. MGI does not make any promise of this manual, including (but not limited to) any commercial of special purpose and any reasonable implied guarantee. MGI has taken measures to guarantee the correctness of this manual. However, MGI is not responsible for any missing parts in the manual, and reserves the right to revise the manual and device, so as to improve the reliability, performance or design. Figures in this manual are all illustrations. The contents might be slightly different from the device, please refer to the device purchased. DNBSEQTM is a trademark of MGI or its subsidiaries in China and/

or other countries. Microsoft and Windows are trademarks or registered trademarks of Microsoft Corporation in the U.S. and/

or other countries. Intel and Intel CoreTM are trademarks of Intel Corporation or its subsidiaries in the U.S. and/or other countries. Other names and brands mentioned in this manual might be claimed as the property of others. 2020-2022 Wuhan MGI Tech Co., Ltd. All rights reserved. Revision date: May 24, 2022 Revision history Date Version Revision May 24, 2022 February 22, 2021 Initial release December 30, 2020 3.0 2.0 1.0 Contents Safety Device overview Conventions General safety Electrical safety FCC statement IC STATEMENT Mechanical safety Components safety Biological safety Symbols Device Label Packaging Intended use Working principle Structural composition Basic components Front view Keyboard and ports Reagent compartment Flow cell Rear view Ports Control software Self-test interface Main interface Menu area I 2 2 4 4 5 6 6 6 7 7 8 9 12 12 13 15 15 17 18 18 19 20 21 21 22 23 s t n e t n o C Getting started Site requirements Network requirements Operating environments of the computer Operation area Log interface Settings interface Maintenance interface Shutdown or restart interface About interface Software security Data and computer ports Access control Installing the device Powering on or off the device Powering on the device Powering off the device Inspecting the device Preparing the power supply Preparing peripheral devices Preparing samples Preparing the reagent cartridge and flow cell Reagent cartridge and sequencing flow cell Washing cartridge and washing flow cell Preparing the pure water container Preparing the waste container Sequencing Workflow Logging in II 25 25 26 27 28 28 30 30 30 31 31 31 31 32 32 32 33 34 34 35 35 35 35 36 37 40 40 C o n t e n t s Disposing of reagent cartridge and flow cell Maintaining the device Selecting a flow cell stage Loading the reagent cartridge Loading the flow cell Reviewing parameters Performing a sequencing run Automatic post-wash Maintaining the main unit Daily maintenance Weekly cleaning Monthly cleaning Maintaining the power supply Maintaining the software Performing a wash Maintaining the pure water container Replacing the waste container Troubleshooting Transportation and storage Disposal of the device Specifications Compliance information Manufacturer information European representative information Index III Appendixes 41 41 41 43 43 44 44 48 48 48 49 49 49 50 51 51 52 53 54 57 59 61 63 65

---This page is intentionally left blank.---Safety This chapter describes basic safety information about the device. Carefully read and understand the information before use to ensure correct operations, best performance, and personnel safety. Keep this manual at hand for reference at any time. 1 y t e f a S Conventions The following table describes conventions that are used in this manual:

Item Description Indicates that the operator should operate the device by following the instructions. Otherwise, it might result Warning in personal injury. Indicates that the operator should operate the device by following the instructions. Otherwise, it might Caution result in device malfunction, damage or inaccurate experiment result. Indicates that the operator should pay special attention to the note information, and operate the device by following the instructions. Indicates biological risks. The operator should operate the device by following the instructions. Indicates the printings and on-screen characters on the Boldface device. General safety Warning z Ensure that the device is operated under the conditions specified in this manual. Otherwise, it might result in incorrect experiment results, device damages, or even personal injury. z Ensure that the components of the device are completely installed before operation. Otherwise, it might result in personal injury. z A laser is installed in the device. Laser radiation can cause eye injury and skin burns. Before performing a sequencing run, ensure that the optics maintenance door, fluidics maintenance door, and flow cell retrieval compartment door of the device are closed. 2 G e n e r a l s a f e t y z Maintain the device by following the instructions in Maintaining the device on Page 47 to ensure best performance. Otherwise, it might result in device malfunction or even personal injury. z Only the technical support authorized by the manufacturer or the qualified and trained personnel can unpack, install, move, and maintain the device. Incorrect operation might cause inaccurate experiment results or damages Caution to the device. z Do not move the device after the technical support have installed and debugged the device. Incorrect operation might result in inaccurate experiment results. If you require to re-position the device, contact the z Only trained personnel, technicians, or laboratory assistants can operate the z Do not operate the device in the presence of flammable or explosive liquids, vapors or gases. Otherwise, it might result in device malfunction, or technical support. device. even personal injury. z Do not disconnect the power cord when the device is on. Otherwise, it might result in device malfunction. z Do not place tubes or reagent kits on the device. Liquids seeping into the device might result in device malfunction. z Do not reuse the disposable items. z Only the peripheral devices and consumables specified by the manufacturer z If you have maintenance questions that are not mentioned in this manual, can be used. consult the technical support. z Only the components provided by the manufacturer can be used for device maintenance. Unapproved components might damage the device or degrade performance. z The device is verified before delivery. If serious deviation occurs during use, contact the technical support for calibration. z A key is needed to open the fluidics maintenance door after the device is powered off and it is only accessible to the technical support authorized by the manufacturer or the qualified and trained personnel, please do not open the flow stage compartment by force. z A screwdriver is needed to open the optics maintenance door after the device is powered off and the door can be opened only by the technical support authorized by the manufacturer or the qualified and trained personnel, please do not open the optics maintenance door by force. 3 y t e f a S Electrical safety Warning z z z z z z Before initial use of the device, assess the electromagnetic environment in which the device will be used. Do not use the device in close proximity to the source of strong electromagnetic fields, such as unshielded sources of radiated emissions. Radiated signals can reduce the accuracy of the results. z Ensure that the device is properly grounded, and the grounding resistance is less than 4 . Failure to do so might result in inaccurate experiment results or even electric shock. Do not remove the device cover and expose the inner components outside. Otherwise, it might cause electric shock. Only the power cord of the manufacturer can be used. Caution Prepare the laboratory and power supply according to Getting started on Page 29. For more details, refer to the relevant site preparation guide. Ensure that the input voltage meets the device requirement. FCC statement This device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions:

1. This device may not cause harmful interference, and 2. This device must accept any interference received, including interference that may cause undesired operation. Any Changes or modifications not expressly approved by the party responsible for compliance could void the users authority to operate the equipment. This equipment should be installed and operated with a minimum distance of 25cm between the radiator and your body. 4 I C s t a t e m e n t This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference in a residential installation. This equipment generates uses and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:

Reorient or relocate the receiving antenna. Increase the separation between the equipment and receiver. Connect the equipment into an outlet on a circuit different from that to which the receiver is connected. Consult the dealer or an experienced radio/TV technician for help. IC statement This device complies with Industry Canadas licence-exempt RSSs. Operation is subject to the following two conditions:

1. This device may not cause interference; and 2. This device must accept any interference, including interference that may cause undesired operation of the device. The distance between user and products should be no less than 20 cm. Le prsent appareil est conforme aux CNR dIndustrie Canada applicables aux appareils radio exempts de licence. Lexploitation est autorise aux deux conditions suivantes:

1. lappareil ne doit pas produire de brouillage, et 2. l u t i l i s a t e u r d e l a p p a re i l d o i t a cce p t e r t o u t b ro u i l l a g e radiolectrique subi, mme si le brouillage est susceptible den compromettre le fonctionnement. La distance entre lutilisateur et de produits ne devrait pas tre infrieure 20 cm. Industry Canada ICES-003 Compliance:CAN ICES-3(B)/NMB-3(B) 5 y t e f a S Mechanical safety Warning Warning Caution To avoid the device falling off and personal injury, place the device on a level surface that meets the load-bearing requirements and ensure that the device cannot be easily moved. Components safety Only the software that has been provided by the manufacturer can be installed and used on the computer. Unknown software might interfere with normal device functions, or even cause data loss. If you need to install antivirus software, contact the technical support in advance. z Do not uninstall the control software by yourself. If any problem occurs during software operation, contact the technical support. z Ensure that the peripheral devices meet the IEC/EN 60950-1 standards. Biological safety z Chemicals in reagents and waste might cause personal injury through contact with the skin, eyes and mucosa. Follow the safety standards of your laboratory and wear protective equipment (such as laboratory coat, and disposable bouffant cap, protective glasses, mask, gloves, and shoe covers) when performing an experiment. z If you accidentally splash the reagent on the skin or into eyes, immediately flush the affected area with large amounts of water and get medical aid z Use and store the reagents according to the reagent kit user manual. Failure to do so might negate the reagent effects and cause inaccurate results. z Check the expiration date of all reagents before use. Do not use expired immediately. reagents. z When disposing of the expired reagents, waste liquid, waste sample and consumables, comply with local regulations. 6 S y m b o l s Symbols Device The following table describes symbols on the device:

Symbol Name Description ON

"ON" (power) supply. Indicates connection to the mains OFF

"OFF" (power) Indicates disconnection from the mains supply. G e n e r a l w a r n i n g sign Signifies a general warning. Warning; biological Warns of a hazard from a biological hazard hazard. Caution; hot surface be hot and should not be touched Indicates that the marked item can without taking care. Warning; dangerous Indicates hazards arising from voltage dangerous voltages. Protective earth p r o t e c t i v e e a r t h ( g r o u n d ) I n d i c a t e s t h e t e r m i n a l o f a electrode. SBC-LAN Network port Connects to the network. BCS-LAN Network port network. C o n n e c t s t h e s e r v e r t o t h e SBC-USB 3.0 USB 3.0 port Connects USB devices such as the keyboard and mouse to the IPC. Connects USB devices such as server. BCS-USB 3.0 USB 3.0 port the keyboard and mouse to the VGA VGA port Used for display adjustment. BCS-Fiber Optical fiber port 10 Gigabit network port. 7 y t e f a S Symbol Name Description RFID identification) cartridge placed near the area. Scans the ID of the flow cell or RFID (Radio frequency reader indication Label The following table describes symbols on the label:

Symbol Name Description Research Use Only

Indicates a device that is for research use only, and cannot be used for clinical diagnosis. Manufacturer Indicates the medical device manufacturer. Date of Indicates the date when the medical manufacture device was manufactured. EC REP Authorized representative in the European Community Indicates the authorized representative in the European Community. RoHS mark EU on the restriction of the use of Indicates that this device meets the requirements of Directive 2011/65/

certain hazardous substances in electrical and electronic equipment. CE mark of conformity Indicates that this device conforms with the specified Council Directive. NRTL Listing and Certification Mark E115163 Used to designate conformance to nationally recognized product safety standards. The Mark bears the name and/or logo of the testing laboratory, product category, safety standard to which conformity is assessed and a control number. 8 Symbol Name Description WEEE symbol collected separately. Please contact S y m b o l s I n d i c a t e s t h a t w a s t e e l e c t r i c a l and electronic equipment must not be disposed of as unsorted m u n i c i p a l w a s t e a n d m u s t b e an authorized representative of the manufacturer for information concerning the decommissioning of your equipment. Indicates the manufacturer's serial SN Serial number number so that a specific medical device can be identified. Consult instructions for use Indicates the need for the user to consult the instructions for use. Packaging The following table describes symbols on the packaging or on the label of the packaging:

Symbol Name Description This way up o f t h e d i s t r i b u t i o n p a c k a g e fo r Indicates the correct upright position transport and/or storage. F r a g i l e , h a n d l e with care carefully. Indicates a medical device that can be broken or damaged if not handled Keep dry Indicates a medical device that needs to be protected from moisture. Do not stack I n d i c a t e s t h a t s t a c k i n g o f t h e distribution package is not allowed and no load shall be placed on the transport package. 9 y t e f a S Symbol Name Description Do not roll shall not be rolled or turned over but Indicates that the transport package shall remain in the upright position. Temperature limit which the medical device can be Indicates the temperature limits to safely exposed. Humidity limitation Indicates the range of humidity to which the medical device can be safely exposed. Atmospheric Indicates the range of atmospheric pressure limitation pressure to which the medical device can be safely exposed. 10 Device overview This chapter describes the intended use, working principle, and structural composition of the device. 11 Intended use The device is a sequencing instrument that measures optical and electronic signals of the reporting molecules to decode the sequence information of a piece of DNA or RNA fragment through the use of instrument specific reagents and flow cells imaging hardware, and data analysis software. The device is intended for decoding the sequence of DNAs or RNAs prepared into specific sequencing libraries such as DNA nanoball libraries. It can be used for whole genome sequencing and de novo sequencing. Warning This device is intended only for scientific research and should not be used for clinical diagnosis. Working principle The device adopts the advanced DNA Nanoball (DNB) and the core technology of combinatorial probe-anchor synthesis (cPAS) and uses a regular arrayed flow cell with the special decorated surface. Each decorated site of the flow cell contains a single DNB, and the decorated site is evenly arranged on the flow cell, ensuring that the optical signal of different Nanoball cannot be interrupted by each other. Therefore, the accuracy of signal process is improved. The following figure demonstrates how to make DNBs:

Replicate DNA Nanoball (DNB) Ad-inserted ss DNA circle N 3 2 1 Figure 1 Making DNBs DNBs w e i v r e v o e c i v e D 12 S t r u c t u r a l c o m p o s i t i o n The following figure demonstrates how to load DNBs:

DNBs Flow cell Decorated sites evenly spread on the flow cell. Each site contains a single DNB. Figure 2 Loading DNBs The DNB and sequencing reagent are pumped into the sequencing flow cell through the devices liquid system. Each DNB combines with the fluorescence group. The laser excites the fluorescence group to emit light, and the optical signals are acquired by the camera. Then the optical signals are processed and transferred to digital signals, the digital signal is transmitted to and processed by the computer, so as to acquire the nucleotide sequence of the DNB. Structural composition The device consists of the main unit and pre-installed control software (software version: V1). The main unit includes the main structure, host, optical system, XYZT-stage, flow cell stage, gas-

liquid system, electric control system, reagent storage system, power supply system, display system, robotic arm, flow cell drive, and flow cell retrieval compartment. The following table describes functions of each component:

Item Description Main structure Provides the stable support for the main unit. Host data. Controls the device, collects, analyzes, and stores Optical system Images the fluorescence signal on the flow cell. XYZT-stage Moves the flow cell and focuses automatically. 13 Item Description Flow cell stage the temperature of the flow cell. Connects the flow cell to fluidics lines and controls Gas-liquid system the biochemical reaction. Provides the gas-liquid support that is required for E l e c t r i c co n t ro l Controls the electric system. system system system Reagent storage Provides the reagent storage environment. P o w e r s u p p l y Provides the power supply for the device. Display system Provides the human-computer interaction interface. Robotic arm Transfers and loads flow cells. Flow cell drive Loads a flow cell for sequencing or washing. Flow cell retrieval compartment Holds used flow cells. w e i v r e v o e c i v e D 14 Basic components Front view 13 13 1 2 3 4 5 6 7 7 Figure 3 Front view No. Name Description 1 2 T o u c h s c re e n Facilitates on-screen operation and displays monitor information. Flow cell drive button Touch to eject the flow cell drive. B a s i c c o m p o n e n t s 12 11 10 9 8 15 No. Name Description 3 4 5 6 7 8 9 Flow cell drive Entry of the flow cell. Tap the flow cell drive button to eject the flow cell drive. Operation panel USB devices in this area, such as the keyboard You can power on or off the IPC, and connect and mouse. Supporting feet Supports the main unit to ensure stability. Caster Used for moving the device. Reagent Holds reagent kits and samples at appropriate compartment temperatures. You can press to open the door. Ventilation inlet Ventilates the device. Waste container port Used for connecting the waste container. Displays the current status of the device:

z Green: the device is running. 10 LED status bar z Blue: the device is in standby status. z Red: an error occurs. z Yellow: a warning appears. 11 Ventilation inlet inlet. Air enters the air filter in the device through this 12 retrieval Retrieves used flow cells. Flow cell compartment 13 maintenance Fluidics door Used by the technical support or trained personnel to maintain the fluidics system. w e i v r e v o e c i v e D 16 Keyboard and ports 1 2 1 3 4 4 3 B a s i c c o m p o n e n t s Figure 4 Keyboard and ports No. Name Description Keyboard tray latch Used to fix the keyboard tray. 2 Keyboard tray Used to support the keyboard and mouse. When not in use, fold the keyboard tray and press it towards the latch until you hear a click. USB 3.0 port keyboard and mouse. C o n n e c t s t o U S B d e v i ce s s u c h a s t h e Host power button Press to power on or off the computer. 17 Reagent compartment The reagent compartments include the sequencing cartridge compartments and washing cartridge compartments. The system automatically identifies the QR code of the reagent cartridges by built-in RFID readers. A B C D 1 2 1 2 A B C D Figure 5 Reagent compartment No. Name Description Sequencing cartridge compartment Washing cartridge compartment H o l d s t h e s e q u e n c i n g c a r t r i d g e s a t appropriate temperatures. Holds the washing cartridges. Flow cell 1 IN Figure 6 Flow cell w e i v r e v o e c i v e D 18 No. Name Description 1 orientation Shows the direction of placing the flow cell. Flow cell marker Rear view 1 2 Figure 7 Rear view 3 No. Name Description 1 Handle Holds it to easily disassemble the rear panel during maintenance. B a s i c c o m p o n e n t s 7 6 5 4 19 No. Name Description Ventilation outlet Ventilates the device. Power inlet Connects to the power cord. Ports Used for cable connections. Pure water container port Connects to the pure water container. Optics Used by the technical support to maintain maintenance door the optical system. Maintenance door button Used for opening the door after unlocking. w e i v r e v o e c i v e D Figure 8 Ports No. Name Description Network port 1 Connects the IPC to the network. Network port 2 Connects the server to the network. USB 3.0 port 1 mouse to the IPC. Connects USB devices such as the keyboard and USB 3.0 port 2 mouse to the server. Connects USB devices such as the keyboard and VGA port Connects to the LCD screen for adjustment. O p t i c a l f i b e r port 10 Gigabit network port. 20 Ports SBC-LAN BCS-LAN SBC-USB3.0 SBC-USB3.0 BCS-USB3.0 VGA BCS-F iber 2 3 4 5 6 7 1 2 3 4 5 6 7 1 2 3 4 5 6 C o n t r o l s o f t w a r e No. Name Description 7 Power switch device. Powers on or off the device:

z Switch to the ON position to power on the z Switch to the OFF position to power off the device. Control software The system control software initiates the communication protocol through physical ports to coordinate with the hardware, control gas lines, fluidics lines, temperature, mechanical components, and optical components. The software detects the signal on the sequencing flow cell, transfers the photographic information to the base sequence files in standard format, and guides different users to perform different processes, such as maintenance and experimental protocols. The following table describes the function of each functional module:

Item Description Self-test functional. Checks whether the components of the system are Sequence Performs different types of sequencing processes. Wash the system. Performs wash and maintenance for fluidics lines of z Because the flow cell stages have the same functions, flow cell stage A is used as an example in the subsequent sections. z For interface control, you can use the touch screen monitor or keyboard and mouse. Self-test interface After you power on the device, self-test starts. If the self-test succeeds, the main interface appears. If the self-test fails, perform the following steps:

1. In the main interface, tap

, and select Log to check the detailed self-test results that are recorded in the log. 21 2. Follow the on-screen instructions or the solutions that are mentioned in Troubleshooting on Page 52. 3. Perform self-test again:

Tap

, select Maintenance > Self-test. Tap

, select Shut down > Restart. If the problem persists, contact the technical support. Main interface The main interface appears after a successful self-test, as shown in the figure below:

3 A B C D L L L L Figure 9 Main interface The following table describes the function of each area or icon in the main interface:

No. Name Description Displays the local date and time. Operation area wash and sequence options when enter the Indicates the status of flow cells and provides main interface of the selected flow cell stage. Menu area operations. P re s s t h e b u t t o n s t o p e r f o r m re l a t i v e 1 2 3 w e i v r e v o e c i v e D 1 2 22 Tap on a flow cell stage name and you can enter the main interface of the selected flow cell stage. A B C D Wash Sequence C o n t r o l s o f t w a r e Figure 10 Main interface A Menu area Item Description The following table describes the function of menu buttons. Tap to return to the main interface when the flow cell stage is not preparing for sequencing or washing. Sensor status indicator. Tap to check the status of sensors for all flow cell stages. A red dot appears on the icon when an error occurs. Tap to view prompt details. The prompt icon includes the following status:

z Yellow: a warning appears. z Red: an error occurs. Tap to log in to the system. Menu button. When the device is in idle or paused statues, you can tap this button to view the system information or logs, or perform system maintenance. Otherwise, you can only tap to view the system information and logs. 23 Item Description After logging into the system, you can tap this button to lock the screen. The following table describes the function of the sensor status indicators in the menu area. Item Description Connection status with ZLIMS. Disk space status. Device temperature status. The real-time value is displayed on the left. Device humidity status. The real-time value is displayed on the left. Waste level status of the built-in waste container. If error indication appears, contact the technical support. The following table describes the function of the sensor status indicators on the main interface of the selected flow cell stage. Item Description Tap to view the status of sensors of the selected flow cell stage. AC T G Zebracall connection status. Fluidics chuck vacuum. The real-time value is displayed on the left. left. Imager vacuum. The real-time value is displayed on the Fluidics chuck temperature. The real-time value is displayed on the left. Sequencing cartridge compartment temperature. The real-time value is displayed on the left. w e i v r e v o e c i v e D 24 Operation area The following table describes the function of icons or information in the area:

Item Description Flow cell stage name. If an error occurs in a flow cell stage, an error indicator appears on the progress bar. xx%

Task progress The flow cell stage is under sequencing. O p e r a t i o n a r e a The fluidics lines of the flow cell stage are under washing. The flow cell stage is in idle status. The flow cell stage is preparing for sequencing or washing. Sequencing or washing is being paused. Sequencing or washing is paused. Sequencing or washing is being stopped. Log interface You can view the log in this interface. To open the log interface, tap Log. in the main interface, and select 25 The following table describes the function of controls in the interface:

Item Description Tap to exit the log interface and return to the previous interface. All Info Tap to view all types of logs. Tap to view information-type logs. Warning Tap to view warning-type logs. Error Tap to view error-type logs. Tap to select the date in the pop-up calendar. Flow cell Select the check box to view the logs of a flow cell. Sort by Set the display order of the logs. Settings interface You can manage recipes and change system settings in this interface. To open the settings interface, log in to your account, tap

, and select Settings. Settings Recipe Language Network Data upload Customize Creation time Sort Recipe SE100+10 SE150+10 PE100+10 PE150+10 Create Import Export Delete Figure 11 Settings interface w e i v r e v o e c i v e D 26 M a i n t e n a n c e i n t e r f a c e Recipe name Read length Dark reaction cycles Read1 Read2 Barcode DualBarcode Read1 Read2 Recipe Customize a recipe Save Figure 12 Recipe interface The following table describes the function of controls in the interface:

Item Description Tap Recipe to enter the Recipe interface. In this interface, you can:

z Tap Sort to change the display order of the recipes. Recipe z Tap Create to customize a recipe. z Tap Import to import recipes. z Tap Export to export customized recipes. z Tap Delete to delete the customized or imported recipes. Tap to change the language of the software. Restart the computer to apply the changes. Input the IP address and port number of the ZLIMS server. Modification takes effect after restarting the computer. Tap to set data processing methods. Language Network Data upload Tap to change the wait time before the screen locks Customize automatically. Move the slider to change the volume of the buzzer. Maintenance interface You can empty the fluidics line and perform self-test in this interface. 27 To open the maintenance interface, log in to your account, tap and select Maintenance.

, The following table describes the function of controls in the interface:

Item Description Cleaning tool replacement Empty fluidics line Device maintenance Tap to replace the cleaning tool. Tap this button and select a flow cell stage to discharge the residual liquid in its fluidics line to the waste container. Tap to perform a self-test for the hardware of the device. The result of After self-test, you will be prompted that the self-test is finished. Tap to unlock the optics maintenance Self-test each item is displayed in the interface. Door control door. Unlock optics maintenance door Only the technical support or trained personnel can maintain the optical Tap to unlock the fluidics maintenance Unlock fluidics maintenance door Only the technical support or trained personnel can maintain the fluidics system. door. system. Shutdown or restart interface You can shut down or restart the computer in this interface. To open the shutdown or restart interface, tap down. and select Shut About interface You can view the software version, serial number, and other information of the device, and the manufacturer information in this interface. To open the About interface, tap and select About. w e i v r e v o e c i v e D 28 Getting started This chapter describes laboratory preparations and sequencing preparations. 29 d e t r a t s g n i t t e G Site requirements Caution z Ensure that the laboratory floor is level and with a gradient of less than z Ensure that the laboratory is free of dust, corrosive and flammable gas, and z Ensure that the laboratory is away from direct sunlight and is well ventilated. We recommend that you refer to the standard of a biosafety 1/200. heat and wind sources. level (BSL) 2 laboratory. z We recommend that you use a clean laboratory with ISO Class 8 air cleanliness. Ensure that air can be circulated inside the laboratory but cannot be circulated to other laboratories. z The device should be used with the ultra-pure water machine, refrigerator, and DNB loader, and can be used with the library preparation system. Therefore, enough space should be considered when placing the above instruments. z Ensure that enough space is provided around the device for ventilation, cable connection, and power switch operation. The following figure indicates distances that are required for optimal operation and access. B Operation area A B B A 1500 mm (59.1 inches) B 1000 mm (39.4 inches) Figure 13 Space requirements Network requirements Operating environments of the computer Minimum configuration Processor: Intel Core i7-7700 30 Software environments Warning Only the software that has been provided by the manufacturer can be installed and used on the computer. Because unknown software might interfere with normal device functions, or even cause data loss. If you need to install antivirus software, contact the technical support in advance. z Software architecture: C/S z Pre-installed software on the computer includes:

Control software Microsoft Windows10 64-bit operating system Microsoft .Net Framework 4.6.1 and above IronPython 2.7.9 Network conditions z Network type: local network z Network bandwidth: no less than 10 Gbit/s Software security I n s t a l l i n g t h e d e v i c e If you need to install antivirus software, contact the technical support in advance. Data and computer ports z Network ports: connects to the network. z USB ports: connect to external USB devices, such as the keyboard and mouse, or for future use. Access control Only the user authorized by the manufacturer can use the control software. Installing the device The device can be installed only by the technical support. And accompanying items such as the keyboard and mouse will be connected. 31 T o e n s u re t h a t t h e p e r fo rm a n ce o f t h e d e v i ce m e e t s t h e specifications, the technical support will perform a standard sequencing run before customer training and use. Powering on or off the device Caution z Ensure that the power switch is in the OFF position before connecting to the power supply. z Ensure that the grounding cable is connected in accordance with the relevant standard or under the guidance of an experienced electrician. z Only the power cord of the manufacturer can be used, and the power cord can be only used with this device. Failure to do so might damage the power cord or device. Powering on the device Perform the following steps:

1. If a UPS is prepared, connect the UPS to the device. 2. Turn the power switch of the device to the ON position. After you power on the device, self-test begins. Powering off the device Before you power off the device, ensure that the sequencing run and wash are completed, the control software is shut down, and the flow cell drive is withdrawn. Failure to do so might damage the device. Perform the following steps:

1. Tap

, select Shut down. In the pop-up dialog box, select Shut down. 2. Turn the power switch to the OFF position. d e t r a t s g n i t t e G 32 Inspecting the device Caution z Only the technical support of the manufacturer or trained personnel can unpack the device. Contact the technical support to unpack and install the device upon delivery. Failure to do so will void the warranty. z Ensure that the outer package is intact and the indicator status of the anti-

shock and anti-tilt label is normal upon delivery. If any problem occurs, contact the technical support. The device contains precise components. Therefore, anti-shock and anti-tilt labels are posted on the outer packaging to monitor any effects of transportation. The following table describes indicator status on the label:

Table 1 Indicator status Label Indicator status Description Remains unaffected d u r i n g t r a n s p o r t a t i o n , o r t h e I n s p e c t i n g t h e d e v i c e A n t i - s h o c k label Red Red Indicates that the device is intact and no strong collision occurs intensity does not exceed the limit. Indicates that the device might not be intact and that a strong collision occurs during transportation and the intensity exceeds the limit. Indicates that no tilt occurs, or Indicates that tilt occurs, and the gradient exceeds the limit. Remains unaffected the gradient does not exceed the Anti-tilt label limit. 33 Preparing the power supply Caution z To ensure a steady and uninterrupted power supply to the device during operation, we recommend the use of a separate UPS. For details about the UPS installation protocol, consult the UPS supplier. z The required power supply of the UPS should be no less than 5 kVA. Connect the power cord of the device to the output port of the UPS. z Ensure that the voltage meets the requirement to prevent poor performance of electric components. Failure to do so might damage electrical components. Table 2 Power specifications Item Description Voltage and frequency range 200 V - 240 V~, 50/60 Hz Voltage fluctuation Rated power 10%

3000 VA Transient over-voltage category II Preparing peripheral devices Table 3 Peripheral devices Device Recommended Remarks supplier The manufacturer The manufacturer DNB loader Sample p r e p a r a t i o n system Ultra-pure water machine General laboratory supplier

Frost-free freezers or

refrigerators Temperature ranges (according to requirements):

z 2 to 8 (36 to 46 ) z -18 to - 25 (-0.4 to -13 ) d e t r a t s g n i t t e G 34 P r e p a r i n g s a m p e s l Preparing samples We recommend that you use the library preparation system of the manufacturer to prepare samples. For details, refer to the relevant user manual. Preparing the reagent cartridge and flow cell Chemicals in reagents and waste might cause personal injury through contact with the skin, eyes, and mucosa. Follow the safety standards of your laboratory and wear protective equipment (such as laboratory coat, and disposable bouffant cap, protective glasses, mask, gloves, and shoe covers) when performing a sequencing run. Reagent cartridge and sequencing flow cell Use only the reagent cartridge and flow cell of the manufacturer for the sequencing process. You can purchase them as needed from the authorized sales representatives. Remove the sequencing reagent cartridge from storage and prepare it for sequencing. For details, refer to relevant reagent kit user manual. After preparing the sequencing reagent cartridge, place it at 2 to 8 (35.6 to 46.4 ). If the sequencing reagent cartridge is not used immediately, store the cartridge at appropriate temperatures as required. Thaw the cartridge thoroughly before performing a sequencing run. Washing cartridge and washing flow cell Washing cartridges are delivered with the device for wash procedures. Used sequencing flow cells function as washing flow cells. For how to prepare the cartridge, contact the technical support. And for how to perform a wash manually, refer to Performing a wash on Page 50. Extra washing reagents are not provided by the manufacturer, but you can purchase them as needed from the authorized sales representatives. 35

Preparing the pure water container 1. Ensure that the pure water contained is sufficient. Warning Insufficient pure water will result in sequencing failure. For details about pure water consumption, refer to the relevant reagent kit user manual. For details about maintaining the pure water container, refer to Maintaining the pure water container on Page 51. 2. Connect the pure water container to the device. 1) Place the fixing plate on the lid and align the holes. Insert the pure water tube into the pure water container through the aligned holes until the tube reaches the bottom of the container. Ensure that the tube goes through the handle, as shown in the figure below. d e t r a t s g n i t t e G Figure 14 Inserting the pure water tube 2) Secure the fixing plate and lid. 2 1 Figure 15 Securing the fixing plate and lid 36 3) Open the airway. Figure 16 Opening the airway 3. If you need to add pure water into the pure water container during sequencing, perform the following steps:

1) Open the lid in position 1 according to the direction indication on the lid. 2) Insert the water output tube of the pure water machine into the pure water container through the water inlet in position 2. 1 2 P r e p a r i n g t h e w a s t e c o n t a i n e r Figure 17 Adding pure water during sequencing 3) Fill the pure water container with fresh pure water. 4) Remove the water output tube of the pure water machine from position 2 and secure the lid in position 1. Preparing the waste container The waste container is connected to the device through the tubes. Ensure that the space is sufficient before connecting the waste container to the device. When the space is insufficient, replace the waste container. 37 For details about estimating the space, refer to the relevant reagent kit user manual. For details about replacing the waste container, refer to Replacing the waste container on Page 51. d e t r a t s g n i t t e G 38 Sequencing This chapter describes the sequencing workflow and washing procedures. Read and follow the instructions to ensure correct operations. 39 Workflow Log in ABCD Select a flow cell stage Load the reagent cartridges and flow cell Review parameters Perform a sequencing run Automatic post-wash z Chemicals in reagents and waste might cause personal injury through contact with the skin, eyes, and mucosa. Follow the safety standards of your laboratory and wear protective equipment (such as laboratory coat, and disposable bouffant cap, protective glasses, mask, gloves, and shoe covers) when performing a sequencing run. z When the waste reaches the specified limit before or during sequencing, dispose of the waste according to local regulations and your laboratory safety standards. Wear protective equipment (such as laboratory coat, and disposable bouffant cap, protective glasses, mask, gloves, and shoe covers) when handling the waste. Personal injury might be caused by contact with the skin, eyes, and mucosa. Logging in to the control software. Perform the following steps:

1. Power on the device. manufacturer. 3. Tap in the main interface. You can perform the sequencing and wash procedures only after you log in 2. Log in to the computer with the password that are provided by the g n i c n e u q e S 40 4. Log in to the control software with the user name and password that are provided by the manufacturer. Selecting a flow cell stage In the operation area of the main interface, select a flow cell stage. Loading the reagent cartridge Perform the following steps:

and washing cartridge. 1. Prepare the reagent cartridges, including the sequencing cartridge For details about preparing the reagent cartridge, refer to the relevant reagent kit user manual. 2. Open the reagent compartment door. 3. Open the sequencing cartridge compartment door by holding its handle and pulling down. Push the sequencing cartridge in the direction of the arrow shown on the cartridge into the reagent compartment until it stops. Close the sequencing cartridge compartment door. 4. Pull out the drawer, put the washing cartridge in the direction shown on the cartridge into the drawer, and push the drawer into the reagent compartment until it stops. 5. Close the reagent compartment door. S e l e c t i n g a f l o w c e l l s t a g e Loading the flow cell Perform the following steps:

1. Tap Sequence and select New run or Resume run, the flow cell drive automatically ejects. Or tap the flow cell drive button on the device to eject the flow cell drive manually. 2. Put a new sequencing flow cell on the flow cell drive according to the direction indication on the flow cell and tap the flow cell drive button to retract. 3. The system automatically identifies the IDs of the flow cell and reagent cartridges. If RFID scanning fails, input the ID with the on-screen keyboard. 41 A B C D Sequencing cartridge ID REF+SN, e.g. 1000000000S0000000000 Washing cartridge ID REF+SN, e.g. 1000000000S0000000000 Flow cell ID xxxxxxxx Recipe SE100+10 1-128 Split barcode Advanced settings A: Preparing Previous Next Figure 18 Sequencing information input interface z Ensure that the ID format is correct when you input ID manually. Otherwise, you will be prompted that the ID is incorrect and the procedure cannot continue. z The ID of the reagent cartridge consists of the 10-digit catalogue number and 11-character serial number. z If the flow cell accidentally drops to the floor and breaks, handle with care in case of personal injury. 4. Tap the Recipe box and select a recipe from the list. To customize a recipe, select Customize a recipe and set the Recipe name, Read length, and Dark reaction cycles. A B C D Customize a recipe Recipe name Read length Dark reaction cycles Read1 Read2 Barcode DualBarcode Read1 Read2 A: Preparing Back Save Figure 19 Customizing a recipe The recipe name must be unique. 5. Select a barcode range from the list on the right of the Recipe box. If you need to import Barcode files, select Import from the list. g n i c n e u q e S 42 6. Tap Advanced settings and you can enable Custom primers and Auto wash if needed. In the sequencing process, you can tap to enable or disable Auto wash if needed. 7. Tap Next. If any error prompts occur, follow the on-screen prompt to solve the problem and tap Next again. Reviewing parameters Carefully check each item in the review interface, and do one of the following:

z If you find any error, tap Previous to return to the previous interface and reset parameters. z If all parameters are correct, tap Start. Ensure that the pure water volumn is sufficient and tap Continue. The software automatically checks whether system modules are working properly. If all modules are working properly, the system automatically starts a sequencing run. If the system prompts that the storage space is insufficient, solve the problem and tap Start to check again. If other problem occurs, contact the technical support. R e v i i e w n g p a r a m e t e r s Performing a sequencing run Warning z Ensure that all compartment doors are closed. Sequencing run cannot be started when the reagent compartment door is open. z Only open the reagent compartment door when necessary to avoid adverse effects on sequencing results or even damage to the device. z Do not bump, move, vibrate, or impact the device during sequencing. Otherwise, sequencing results might be inaccurate. z Pay special attention to the LED status bar, icons, and prompts. If errors occur, a message appears on the screen. Follow the prompt to troubleshoot the problem. For information about the troubleshooting, refer to Troubleshooting on Page 52. 43 The following table describes the function of each item in the interface:

Item Description Estimated completion time QC type Shows the estimated time when sequencing completes. You can select a QC value graph from the QC type list to assess the sequencing quality. Tap it and a confirmation dialog box appears. Select Yes to pause sequencing. Tap it again and a confirmation dialog box appears. Select Yes to resume sequencing. Tap it and a confirmation dialog box appears. Select Yes to stop sequencing. Tap to view the First Base Report. T a p t o v i e w t h e s u m m a r y a f t e r a s e q u e n c i n g r u n Tap to view sequencing information after a sequencing run completes. starts. Automatic post-wash Auto wash is enabled by default. The system automatically performs a post-wash after each sequencing run. During troubleshooting, you can set Auto wash to No when n e ce s s a r y a n d p e r fo r m a w a s h m a n u a l l y r i g h t a f t e r t h e troubleshooting. For details about how to perform a wash manually, refer to Performing a wash on Page 50. Disposing of reagent cartridge and flow cell Warning After sequencing and post-wash, recycle the used flow cells from the flow cell retrieval compartment. Before powering off the device, check whether the used flow cells are recycled. After sequencing and post-wash, or before powering off the device, perform the following steps:

1. Wear protective equipment. g n i c n e u q e S 44 2. Open the flow cell retrieval compartment and remove the flow cell. Used sequencing flow cells function as washing flow cells. The washing flow cell can be reused for three times. Dispose of the flow cell according to local regulations and safety standards of your laboratory. 3. Open the reagent compartment door and remove the reagent cartridges. 4. Empty the remaining solution in the reagent cartridge into an appropriate waste container. 5. Dispose of the flow cell and reagent cartridges in accordance with local regulations and safety standards of your laboratory. D i s p o s i n g o f r e a g e n t c a r t r i d g e a n d f l o w c e l l 45

---This page is intentionally left blank.---Maintaining the device This chapter describes maintenance procedures of the device and its parts. Perform maintenance regularly to ensure that the device runs smoothly. 47 Maintaining the main unit Daily maintenance z Perform the following maintenance when the device is powered on:

Check whether the fan at the rear of the device is operational. If not, contact the technical support. During sequencing, pay attention to error messages and check whether the relevant parts can work properly. Contact the technical support if needed. For information about how to find error messages, refer to Menu area on Page 23, and Log interface on Page 25. z Clean the interior of the reagent compartment before sequencing. 1) Power off the device, open the reagent compartment, open the sequencing cartridge compartment door and pull out the washing cartridge drawer. For details about powering off the device, refer to Powering off the device on Page 32. 2) Clean the interior of the reagent compartment with a 75%

alcohol wipe. Ensure that the surface is free of DNBs, reagents, blood, and saliva. Weekly cleaning Warning z We do not recommend that you use other disinfectants or wash solutions except for those that are mentioned in this manual. Because other solutions are not verified for use and their effects to the device are unknown. z If you have questions about the compatibility of wash solutions, contact the technical support. Perform the following steps:

1. Power off the device and open the reagent compartment. For details about powering off the device, refer to Powering off the device on Page 32. 2. Clean the touch screen and exterior of the reagent compartment door, sequencing cartridge compartment door, and washing cartridge drawer with a 75% alcohol wipe. Ensure that the surface is free of DNBs, reagents, blood, and saliva. e c i v e d e h t g n n i a t n i a M i 48 Monthly cleaning Warning z We do not recommend that you use other disinfectants or wash solutions except for those that are mentioned in this manual. Because other solutions are not verified for use and their effects to the device are unknown. z If you have questions about the compatibility of wash solutions, contact the technical support. Perform the following steps:

1. Power off the device and open the reagent compartment. For details about powering off the device, refer to Powering off the device on Page 32. 2. Clean the surface of the device with a 75% alcohol wipe. Ensure that the surface is free of DNBs, reagents, blood, and saliva. Maintaining the power supply z When the device is not in use for seven days or longer, perform a wash manually according to the relevant reagent kit user manual. Turn off the device after the wash. For details about powering off the device, refer to Powering off the device on Page 32. z Check whether the power cord and cables are in good condition regularly. Contact the technical support if new cables are required. Maintaining the software After the receival of Release Notification, contact the technical support to update the software. i M a i n t a i n n g t h e p o w e r s u p p l y 49 Performing a wash Chemicals in reagents and waste might cause personal injury through contact with the skin, eyes, and mucosa. Follow the safety standards of your laboratory and wear protective equipment (such as laboratory coat, and disposable bouffant cap, protective glasses, mask, gloves, and shoe covers) when performing a sequencing run. Warning z We do not recommend that you use other disinfectants or wash solutions except for those that are mentioned in this manual. Other solutions are not verified for use and their effects to the device are unknown. z If you have questions about the compatibility of wash solutions, contact the technical support. You can perform a wash to remove the remaining reagents from the fluidics lines and flow cell stages, and avoid cross-contamination. When Auto wash is enabled, the system automatically performs a wash after each sequencing run. If Auto wash is set to No or the device is not used for 7 days or longer, perform a wash manually. Wash solutions that can be used include:

z 1 M NaCl and 0.05% Tween-20 z 0.1 M NaOH z Laboratory-grade water You can use laboratory-grade water such as 18 Megohm (M) water, Milli-Q water, Super-Q water, or similar molecular biology-grade water. 1. Before sequencing, log in to your account. Tap Wash in the main Perform the following steps:

interface of the flow cell stage. 2. Prepare the washing cartridge. For details about preparing the reagent cartridge, refer to the relevant reagent kit user manual. 3. Follow the on-screen steps to load the washing flow cell, washing cartridge, and an empty sequencing reagent cartridge. Close all compartment doors. For details, refer to Loading the flow cell on Page 41 and Loading the reagent cartridge on Page 41. 4. In the wash interface, tap Wash and a confirmation dialog box appears. Select Yes to start wash. Tap to pause, and tap it again to resume wash. e c i v e d e h t g n n i a t n i a M i 50 Tap and a confirmation dialog box appears. Select Yes to stop wash. cartridge. 5. After wash, tap Finish to return to the main interface. 6. Remove the washing flow cell, sequencing cartridge, and washing 7. Store the washing flow cell at room temperature. The washing flow cell can be reused for only one month. Dispose of the flow cell according to local regulations and safety standards of your laboratory. 8. Empty the remaining solution in the washing cartridge into an appropriate waste container. 9. Dispose of the washing cartridge according to local regulations and safety standards of your laboratory. M a i n t a i n i n g t h e p u r e w a t e r container Perform the following maintenance every week:

1. Empty the pure water container. 2. Spray 75% alcohol onto the inner surface of the lid and the surface of the pure water tube, and then wipe them with a clean cloth. 3. Add sufficient laboratory-grade water into the pure water container, and attach the lid back onto the container. 4. Gently swirl it until all inner walls are cleaned. 5. Empty the pure water container. 6. Repeat steps 3 to 5 twice. Replacing the waste container Chemicals in reagents and waste might cause personal injury through contact with the skin, eyes, and mucosa. Wear protective equipment (such as laboratory coat, and disposable bouffant cap, protective glasses, mask, gloves, and shoe covers) when handling the waste. Personal injury might be caused by contact with the skin, eyes, and mucosa. i M a i n t a i n n g t h e p u r e w a t e r c o n t a i n e r 51 The waste container is connected to the device through tubes. To avoid liquid leakage and biological hazard, monitor the waste container status frequently and dispose of the waste and waste container in time when the waste reaches the maximum volume. Maximum volume Figure 20 Maximum waste volume Perform the following steps:

1. Wear protective equipment. 2. Remove the lid with tubes from the waste container, install a new lid with sealing gasket, and secure the lid until you hear a click. 3. Dispose of the waste and waste container according to local regulations and safety standards of your laboratory. Troubleshooting If malfunction occurs during operation, the device beeps or a message is displayed on the screen. Follow the prompt to troubleshoot and solve the problem. The following table lists some of the problems and possible solutions. If you need additional assistance, contact the technical support. Table 4 Troubleshooting Problem Possible cause Recommended action After turning the power switch to ON position, I cannot turn on the device. z The mains supply 1. Check whether the mains is abnormal. supply and UPS is normal. z The device is not 2. Ensure that the device is connected to the connected to the mains mains supply or supply or UPS. UPS. 3. If the problem persists, z The UPS runs out contact the technical of power. support. e c i v e d e h t g n n i a t n i a M i 52 Problem Possible cause Recommended action Error messages appear when the control software runs. z The parameters are not set properly. z Errors occur when the software communicates with hardware. 1. Perform a self-test in the maint enance int er f ace. Check the record of the hardware that fails the self-

test. 2. C h e c k e r ro r m e s s a g e s in the log, and solve the problem according to on-

screen instructions. 3. Restart the device. 4. If the problem persists, c o n t a c t t h e t e c h n i c a l support. Temperature error z The temperature message and warning appears e x c e e d s t h e Record the warning and logs preset limits. of this run and contact the in the sequencing z Temperature technical support. interface. sensor error. Waste level sensor z The level sensor alarms. Record the warning and logs of this run and contact the technical support. z The waste level e x c e e d s t h e preset limits. is not installed properly. z The level sensor is damaged. Transportation and storage z For information about the environmental requirements for storing the device, refer to Specifications on Page 57. z If you want to move or transport the device, contact the technical support. T r a n s p o r t a t i o n a n d s t o r a g e 53 Disposal of the device The service life of this device is seven years, which is determined by the simulated service life evaluation method. For the date of manufacture, refer to the label on the device. Perform the maintenance according to the requirements mentioned in this manual. Dispose of the end of life device according to local regulations. Or, if the device is confirmed that it still can work safely and effectively after maintenance, keep the device. e c i v e d e h t g n n i a t n i a M i 54 Appendixes This chapter provides additional important information about the device. 55

---This page is intentionally left blank.---Specifications Item Description Laser classification of the device Class 1 laser product Dimensions 1656 mm (W) 1815 mm (H) 903 mm (D)

(65.2 inches 71.5 inches 35.6 inches ) Net weight Approximately 765 kg (1687 lb) Touch screen Type Size LCD 20 inches Resolution 1920 1080 pixels Voltage 200 V to 240 V~

Frequency 50/60 Hz Rated power 3000 VA Overvoltage category II min.10AWG S p e c i f i c a t i o n s Power Cable 75 dB Maximum sound pressure level Degrees of protection provided by IPX0 enclosures (IP Code) A c c o m p a n y i n g items Refer to the packing list 57 Item Description Temperature 19 to 25 (66 to 77 ) Relative humidity condensing 30% RH to 80% RH, non-

Atmospheric pressure Altitude 80 kPa to 106 kPa 2000 m Operating environment requirements Pollution degree 2 Indoor use Because the temperature and humidity fluctuations influence the accuracy of the experiment results, we recommend that you install an air conditioning system and a humidifier or dehumidifier in the laboratory to maintain the temperature and humidity. Transportation/

Storage environment requirements Temperature

-20 to 50 (-4 to 122 Relative humidity condensing 1 5 % R H t o 8 5 % R H , n o n -

Atmospheric pressure 80 kPa to 106 kPa s n o i t a c i f i c e p S 58 Compliance information The device complies with the following standards:

Item Standard IEC 61326-1:2020 Electromagnetic Compatibility

(EMC) Electrical equipment for measurement, control and laboratory use EMC requirements Part 1:

General requirements z IEC 61010-1:2010+AMD1:2016 Safety requirements for electrical equipment for measurement, control, and laboratory use-

Part 1: General requirements z IEC 61010-2-081:2019 Safety requirements for electrical equipment for measurement, control and laboratory use - Part 2-081: Particular requirements for automatic and semi-automatic laboratory equipment for analysis and other purposes z IEC 61010-2-010:2019 Safety requirements for electrical equipment for measurement, control and laboratory use - Part 2-010: Particular requirements for laboratory equipment for the heating of materials z IEC 60825-1:2014 Safety of laser product part 1: equipment classification and requirements C o m p l i a n c e i n f o r m a t i o n Safety requirements 59

---This page is intentionally left blank.---Manufacturer information Manufacturer Wuhan MGI Tech Co., Ltd. Address Building 24, Stage 3.1, BioLake Accelerator, No.388, 2nd Gaoxin Road, East Lake High-

Tech Development Zone, 430075, Wuhan, P.R.China Technical support Wuhan MGI Tech Co., Ltd. Technical support E-mail MGI-service@mgi-tech.com M a n u f a c t u r e r i n f o r m a t i o n 61

---This page is intentionally left blank.---European representative information Name Latvia MGI Tech, SIA Address Dzirnavu street 57a-4, Rga, Latvia, LV-1010 E u r o p e a n r e p r e s e n t a t i v e i n f o r m a t i o n 63

---This page is intentionally left blank.---Index anti-shock and anti-tilt labels 33 antivirus software 31 Biological safety 6 combinatorial probe-anchor synthesis (cPAS) 12 I n d e x A B C E F H L empty the fluidics line 27 Flow cell 18 Flow cell drive 16 Flow cell drive button 15 Flow cell retrieval compartment 16 Host power button 17 LED status bar 16 65 M N P R S T W Main interface 22 Maintenance 27 Network requirements 30 Performing a wash 50 Washing cartridges 35 peripheral devices 34 Pollution degree 58 Power switch 21 safety information 1 Self-test 21 sensor status indicators 24 Settings interface 26 Troubleshooting 52 Workflow 40 Reagent cartridge and sequencing flow cell 35 Reagent compartment 18 x e d n I 66

D C B A 6 5 4 3 2 1 REV. ECR#

DETAILED DESCRIPTION 4.0 3.0 ECR-00-21003 ICFCC ECR-02-20093 MODIFIER DATE 2022.05.23 2021.02.17 105.0 DNBSEQ-T7RS P roduct Name Genetic S equencer Model No. Supply Voltage 200-240V~,50/60Hz Rated Power 3000VA IC:28537-DNBSEQT7RS FCC ID:2A6TQDNBSEQ-T7RS Research Use Only 0

. 5 7 Wuhan MGI Tech Co., Ltd. Building 24,Stage 3.1,BioLake Accelerator,No.388,2nd Gaoxin Road, East Lake High-Tech Development Zone,430075,Wuhan,P.R.China Latvia MGI Tech, SIA Dzirnavu street 57a-4, Rga, Latvia, LV-1010 YYYY-MM 000000000000 MET C U S E115163 4-R2 D C B 1 SN 2

,Noto_Sans;SN CODE 128,) 3Pantone black c 4Noto_Sans;

5HSF. This
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6 5 4 3 2 DESIGN CHECK CHECK CHECK APPROVAL MATERIAL MGI Tech Co., Ltd. TITLE T7-RS DWG NO. M-021-000291-00 72826

(Must conform to RoHS) FINISH UNIT mm SCALE SIZE 1:2 A4 SHEET 1 OF 1 A REV. 4.0 1

2022-06-06 Equipment Autorisation Division Federal Communications Commission 7435 Oakland Mills Road Columbia, MD 21046 FCC ID: 2A6TQDNBSEQ-T7RS Product Name: Genetic Sequencer Request for Confidentiality Pursuant to Sections 0.457 and 0.459 of the commissions rules, we hereby request that the following documents be held confidential:

Schematics Block diagram Operation description BOM These materials contain trade secrets and proprietary information and are not customarily released to the public. The public disclosure of this information might be harmful to the company and provide unjustified benefits to our competitors. By:

yanxia xie Printed Signature Title: Manager On behalf of :

Telephone: 13560254770 Wuhan MGI Tech Co., Ltd.